| 花生根细胞悬浮培养制备白藜芦醇条件的优化 |
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| 引用本文： | 黄卫文,黎继烈,戴梓茹,李忠海.花生根细胞悬浮培养制备白藜芦醇条件的优化[J].食品科学,2011,32(7):233-236. |
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| 作者姓名： | 黄卫文 黎继烈 戴梓茹 李忠海 |
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| 作者单位： | 1.中南林业科技大学食品科学与工程学院 2.中南林业科技大学生命科学与技术学院 3.钦州学院化学化工学院 |
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| 基金项目： | 湖南省教育厅科技项目(05C335) |
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| 摘 要： | 以花生根愈伤组织为材料，采用二次通用旋转组合试验设计，优化花生根细胞悬浮培养产白藜芦醇的条件，探讨影响花生根细胞悬浮培养产白藜芦醇的最佳工艺参数。当摇床转速为130r/min、接种量为38.00g/L、pH5.80、蔗糖质量浓度为40.00g/L时，培养15d，花生根细胞增殖倍数最大为(4.71±0.04)倍，白藜芦醇产量达到907.3μg/g。
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| 关 键 词： | 二次通用旋转组合设计 花生根细胞 悬浮培养 白藜芦醇 |
| 收稿时间： | 2010-06-07 |
Optimization of Suspension Culture Conditions for Preparing Resveratrol in Peanut Root Cells |
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| HUANG Wei-wen,LI Ji-lie,DAI Zi-ru,LI Zhong-hai.Optimization of Suspension Culture Conditions for Preparing Resveratrol in Peanut Root Cells[J].Food Science,2011,32(7):233-236. |
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| Authors: | HUANG Wei-wen LI Ji-lie DAI Zi-ru LI Zhong-hai |
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| Affiliation: | 1. College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China；
2. College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China；
3. College of Chemistry and Chemical Engineering, Qinzhou University, Qinzhou 535000, China |
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| Abstract: | Quadratic rotation combinatorial design was utilized to optimize suspension culture conditions for resveratrol production by peanut root callus tissue. Under the following optimized conditions: shaker rational speed 130 r/min; inoculum size 38.00 g/L; pH 5.80; and sucrose concentration 40.00 g/L, the maximum proliferation fold of suspension cells was 4.71 ± 0.04, and the resveratrol production reached 907.3 μg/g after 15-day incubation. |
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| Keywords: | peanut root cell callus suspension culture resveratrol |
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