| 5种葡萄球菌肠毒素基因MPCR-DHPLC检测方法的建立 |
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| 引用本文： | 陈 彬,郑 晶,王 颖,黄晓蓉,林 杰,彭华毅,邵碧英.5种葡萄球菌肠毒素基因MPCR-DHPLC检测方法的建立[J].食品科学,2014,35(24):243-248. |
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| 作者姓名： | 陈 彬 郑 晶 王 颖 黄晓蓉 林 杰 彭华毅 邵碧英 |
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| 作者单位： | 1.福建出入境检验检疫局，福建 福州 350001；2.福建省检验检疫技术研究重点实验室，福建 福州 350001；;
3.福建农林大学食品科学学院，福建 福州 350002 |
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| 基金项目： | 福建省科技厅自然科学基金项目（2012J01062）；福建局科技项目（FK2012-25） |
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| 摘 要： | 设计合成5种葡萄球菌肠毒素基因(SEA、SEB、SEC、SED、SEE)的特异性引物,对聚合酶链式反应(polymerase chain reaction,PCR)的反应体系进行优化后,建立5种葡萄球菌肠毒素基因的多重PCR结合变性高效液相色谱(multiplex PCR-denaturing high performance liquid chromatography,MPCR-DHPLC)检测方法,并进行MPCR-DHPLC检测特异性及灵敏度的测定,5重PCR-DHPLC检测灵敏度可达到100 CFU/m L。MPCR-DHPLC方法应用于食品中金黄色葡萄球菌肠毒素的检测,具有快速、准确、高通量等优点。
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| 关 键 词： | 金黄色葡萄球菌 肠毒素 多重聚合酶链式反应 变性高效液相色谱 |
Development of a Multiplex PCR-DHPLC Detection Method for Five Staphylococcal Enterotoxin Genes |
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| CHEN Bin,ZHENG Jing,WANG Ying,HUANG Xiao-rong,LIN Jie,PENG Hua-yi,SHAO Bi-ying.Development of a Multiplex PCR-DHPLC Detection Method for Five Staphylococcal Enterotoxin Genes[J].Food Science,2014,35(24):243-248. |
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| Authors: | CHEN Bin ZHENG Jing WANG Ying HUANG Xiao-rong LIN Jie PENG Hua-yi SHAO Bi-ying |
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| Affiliation: | 1. Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China;
2. Fujian Provincial Key Laboratory of Inspection and Quarantine Technology Research, Fuzhou 350001, China;
3. College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | Primer pairs specific for the five staphylococcal enterotoxin genes SEA, SEB, SEC, SED, and SEE were designed
and synthesized. Using multiplex polymerase chain reaction coupled with denaturing high performance liquid chromatograph
(MPCR-DHPLC), a detection method for these five staphylococcal enterotoxin genes were established after the polymerase
chain reaction (PCR) reaction conditions were optimized. The sensitivity and specificity of the detection method were
then determined. The results showed that the quintuplex PCR-DHPLC method could detect specifically staphylococcal
enterotoxin genes with detection limit of 100 CFU/mL. The MPCR-DHPLC method is useful for the rapid, accurate and
high-throughput detection of staphylococcal enterotoxins in food samples. |
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| Keywords: | Staphylococcus aureus enterotoxin MPCR DHPLC |
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