酱香大曲中链霉菌菌株FBKL4.005全基因组测序及序列分析

链霉菌FBKL4.005是本课题组前期从酱香大曲中分离得到的1株耐高温菌株,能够在37~55℃的范围内生长代谢,为了能够更深入地探究该菌株在酿酒过程中的代谢机理和功能性作用,完成大曲极端微生物开发应用,对于菌株的序列信息进行解析是最为全面高效的方式。本研究采用Illumina HiSeq 2000测序平台对菌株FBKL4.005进行全基因组测序,共得到67 771 429个reads,2.47 G的数据量,通过对测序数据进行拼接,得到69个contigs,菌株整个基因组大小为9 454 406 bp,GC含量为73.03%,经过滤处理后得到7.33Mb的有效数据量及7 946个注释基因,并通过GO、COG、KEGG、NR、TCDB数据库的比对分析,完成菌株基因组预测与基本功能的注释,获得的基因注释信息为今后深入开展酱香大曲中耐高温特性链霉菌类群代谢途径及机理的研究提供了理论支持。

Whole Genome Sequencing and Sequence Analysis of Streptomyces sp. FBKL4.005 from Moutai-Flavor Daqu

Streptomyces sp. FBKL4.005 was a heat-resistant strain isolated from Moutai-flavor Daqu, which could grow and metabolize at 37–55 ℃. In order to get insights into the metabolic mechanism and functional role of the strain in liquor-making for the exploitation and application of extremophiles in Daqu, whole genome sequencing of strain FBKL4.005 was performed on the Illumina HiSeq 2000 sequencing platform. A total of 67 771 429 reads and 2.47 G data were obtained. By de novo sequence splicing, 69 contigs were also acquired. The complete genomic size of stain was 9 454 406 bp with a GC content of 73.03%. After filtration treatment, 7.33 Mb effective data and 7 946 annotated genes were acquired. We conducted a comparative analysis among the GO, COG, KEGG, NR and TCDB database for genomic prediction and functional annotation. The acquirement of gene annotation information can provide an important foundation for further research on the metabolic pathways and mechanism of heat resistant Streptomyces sp. in Moutai-flavor Daqu.