鲍内脏多糖体内抗氧化及增强小鼠免疫活性

为研究鲍内脏多糖(abalone visceral polysaccharides,AVP)体内抗氧化及增强小鼠免疫活性作用,采用乙醇氧化损伤模型动物实验设计和正常小鼠增强小鼠免疫力实验设计,通过测定实验小鼠肝脏超氧化物歧化酶(superoxidedismutase,SOD)活力,谷胱甘肽(glutathione,GSH)、丙二醛(malondialdehyde,MDA)和蛋白质羰基含量综合评价AVP的体内抗氧化能力;通过测定正常实验小鼠免疫器官指数、足跖增厚、溶血素抗体水平、吞噬指数α,从免疫器官指数、细胞免疫、体液免疫以及非特异性免疫方面综合评价AVP增强小鼠免疫活性能力。结果表明:AVP显著提高氧化损伤模型小鼠肝脏SOD活力和GSH含量(P0.05),显著降低小鼠肝脏MDA含量和蛋白质羰基含量(P0.05),表现出良好的体内抗氧化能力;AVP能极显著提高正常小鼠脾脏指数和胸腺指数、足跖增厚和血清溶血素水平(P0.01),显著提升小鼠吞噬指数(P0.05),在免疫器官指数、细胞免疫、体液免疫以及非特异性免疫4个方面均表现出较好的增强小鼠免疫活性效果;另外,数据表明AVP体内抗氧化及增强小鼠免疫活性作用与其剂量呈正相关性。鲍内脏多糖是一种具有良好的抗氧化和增强免疫活性的物质,为鲍内脏的精深加工与产业化开发提供了理论依据。

In Vivo Antioxidant Capacity and Immunoenhancing Activity in Mice of Polysaccharides from Abalone Viscera

The objective of this work was to explore the in vivo antioxidant capacity of abalone viscera polysaccharides (AVP) and its immunoenhancing activity in mice. An animal model of ethanol-induced oxidative injury and normal mice were used for this experiment. The in vivo antioxidant capacity was evaluated by measuring superoxide dismutase (SOD) activity, and the contents of glutathione (GSH), malondialdehyde (MDA) and protein carbonyl content in liver. The immunoenhancing activity was determined with respect to immune orange indices, cell immunity, humoral immunity and nonspecific immunity. For this purpose, we measured immune organ indices, footpad swelling, hemolysin antibody level and phagocytic index (α). Results showed that AVP exerted good in vivo antioxidant capacity by significantly increasing SOD activity and GSH level (P < 0.05), and reducing the contents of MDA and protein carbonyl (P < 0.05) in the liver of mice with ethanol-induced oxidative injury. Furthermore, AVP enhanced immune function in normal mice by significantly increasing spleen and thymus indices, footpad swelling, serum hemolysin level (P < 0.01) and α value (P < 0.05). Additionally, our data demonstrated that both the antioxidant capacity and immunoenhancing activity of AVP were concentration dependent. This study can provide a theoretical basis for intensive processing and industrial development of abalone viscera.