大豆过敏原11S球蛋白G2中A2链结合表位的预测

采用生物信息学软件NCBI Gen Bank数据库、PDB数据库、SWISS-MODEL、Deep View 4.1等预测11S球蛋白G2中A2链的二级结构和三级结构模型,使用Disco Tope 2.0 Server软件预测B细胞构象表位,发现构象表位多位于无规则卷曲处。定位B细胞线性结合表位,结果表明11S球蛋白G2中A2链的可能线性表位有:~(37)KPDNRIE~(43)、~(79)PSYTNGP~(85)、~(114)SQQRGRSQRP~(123)、~(52)WNPNNK~(57)、~(196)QQGGSQSQKGKQQE~(209)、~(241)QGENEEED~(248)、~(267)RKPQQEEDDDDEEEQ~(281)、~(287)TDKGC~(291)。此表位预测为后续制肽、免疫等操作找出更准确的肽序,以便建立适合我国大豆过敏人群的食品大豆过敏原的检测技术。

Mapping of IgG-Binding Epitopes on the A2 Chain of the Major Soybean Allergen 11S Globulin G2

Adopting bioinformatics software, this study aimed to predict the secondary and tertiary structure model of the A2 chain of 11S globulin G2 in order to locate the B cell epitopes. The results indicated that the conformational epitopes were mainly at the random coil areas and the probable linear epitopes may contain 37KPDNRIE43, 79PSYTNGP85, 114SQQRGRSQRP123, 52WNPNNK57, 196QQGGSQSQKGKQQE209, 241QGENEEED248, 267RKPQQEEDDDDEEEQ281, and 287TDKGC291. This prediction can be utilized to find out accurate peptides sequences for subsequent peptide preparation and immunization so as to develop a technique to detect soybean allergens for people allergic to soybean foods in China.