| 沙门氏菌核酸检测试剂盒的评价 |
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| 引用本文： | 邵碧英,董 建,陈 彬,陈文炳,黄晓蓉,郑 晶,缪婷玉,彭 娟.沙门氏菌核酸检测试剂盒的评价[J].食品科学,2015,36(24):242-245. |
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| 作者姓名： | 邵碧英 董 建 陈 彬 陈文炳 黄晓蓉 郑 晶 缪婷玉 彭 娟 |
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| 作者单位： | 福建出入境检验检疫局 福建省检验检疫技术研究重点实验室，福建 福州 350001 |
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| 基金项目： | 福建省科技计划项目（2011Y0002）；国家认监委出入境检验检疫行业标准制（修）订计划项目（2013B264K） |
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| 摘 要： | 采用室内技术参数验证和协同实验的方式对商品化沙门氏菌核酸检测试剂盒在食品中沙门氏菌核酸检测上的包容性、排他性、灵敏度、特异性、准确度、检测限、耐变性、批间变异进行评价。评价结果表明,试剂盒检测结果与国家标准方法检测结果总体上无显著差异,能满足食品样品的检测要求;但对蔬菜制品的灵敏度较其他食品低,与国家标准方法有显著差异,因此该商品化试剂盒不适用于蔬菜制品中沙门氏菌核酸的检测。
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| 关 键 词： | 沙门氏菌 核酸检测试剂盒 环介导等温扩增 评价 |
Evaluation of Salmonella Nucleic Acid Detection Kit |
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| SHAO Biying,DONG Jian,CHEN Bin,CHEN Wenbing,HUANG Xiaorong,ZHENG Jing,MIAO Tingyu,PENG Juan.Evaluation of Salmonella Nucleic Acid Detection Kit[J].Food Science,2015,36(24):242-245. |
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| Authors: | SHAO Biying DONG Jian CHEN Bin CHEN Wenbing HUANG Xiaorong ZHENG Jing MIAO Tingyu PENG Juan |
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| Affiliation: | Fujian Provincial Key Laboratory of Inspection and Quarantine Technology Research,;
Fujian Province Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China |
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| Abstract: | The inclusiveness, exclusivity, sensitivity, specificity, accuracy, limit of detection (LOD), resistance
to denaturation and batch variation of the commercial Salmonella nucleic acid detection kit used to detect Salmonella in food
samples were evaluated based on indoor technical parameters by validation and collaboration test. Results showed that the
kit was not significantly different from the national standard method in general, and could meet the requirements of food
sample testing. However, the sensitivity for vegetables products was lower than those of other foods, which significantly
differed from the national standard method. Thus the commercial kit was not suitable for detection of Salmonella in
vegetable products. |
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| Keywords: | Salmonella nucleic acid detection kit loop-mediated isothermal amplification evaluation |
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