| 禽肉制品中单增李斯特菌的DiversiLab分型 |
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| 引用本文： | 郑 晶,唐中伟,陈 彬,黄晓蓉,林 杰,张体银.禽肉制品中单增李斯特菌的DiversiLab分型[J].食品科学,2015,36(24):220-223. |
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| 作者姓名： | 郑 晶 唐中伟 陈 彬 黄晓蓉 林 杰 张体银 |
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| 作者单位： | 1.福建省检验检疫技术研究重点实验室，福建 福州 350001；2.福建农林大学食品科学学院，福建 福州 350002 |
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| 基金项目： | 福建省自然科学基金项目（2010J01266）；国家质检总局科技计划项目（2010IK169） |
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| 摘 要： | 目的：研究从同一类食品中分离出的单增李斯特菌间的同源性关系，为预测预警保障食品安全和食源性疾病溯源提供科学依据和技术支持。方法：应用DiversiLab分型系统对2001-2010年福建出入境检验检疫局从各类禽肉制品中分离出的25 株单增李斯特菌进行分型，其中包括：DNA提取、重复序列-聚合酶链式反应（repetitivesequence-based polymerase chain reaction，rep-PCR）、微电泳芯片分离检测及数据分析。结果：DiversiLab分型中，相似系数为95%时，25 株菌被分为了6 组（G）；相似系数为97%时，25 株菌被分为11 组（P）。结论：DiversiLab分型结果较准确地揭示了25 株菌株间的亲缘性关系。基于rep-PCR的DiversiLab分型系统，是一种操作简单、分辨率高、重复性好且高效快速的基因分型方法，可作为食源性病原体检测及食源性疾病溯源的工具。
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| 关 键 词： | DiversiLab分型系统 单增李斯特菌 重复序列PCR 同源性分析 |
DiversiLab Typing of Listeria monocytogenes Isolated from Poultry Products |
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| ZHENG Jing,TANG Zhongwei,CHEN Bin,HUANG Xiaorong,LIN Jie,ZHANG Tiyin.DiversiLab Typing of Listeria monocytogenes Isolated from Poultry Products[J].Food Science,2015,36(24):220-223. |
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| Authors: | ZHENG Jing TANG Zhongwei CHEN Bin HUANG Xiaorong LIN Jie ZHANG Tiyin |
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| Affiliation: | 1. Fujian Province Key Laboratory of Inspection and Quarantine Technology Research, Fuzhou 350001, China;
2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | To study the homologous relationship of Listeria monocytogenes strains isolated from poultry products, which
can provide a scientific basis for forecasting and early warning of food security and provide technical support for preventing
food-borne diseases caused by L. monocytogenes. Methods: A total of 25 strains of L. monocytogenes isolated from the
poultry products in the Fujian Entry-Exit Inspection and Quarantine Bureau from 2001 to 2010 were typed by DiversiLab
typing system through DNA extraction, repetitive sequence-based polymerase chain reaction (rep-PCR), and microfluidic
chip. Results: At the similarity coefficient of 95% and 97%, 25 strains were accordingly divided into 6 groups (G) and
11 patterns (P), respectively. Conclusions: The genetic relatedness among the 25 strains could be accurately revealed by
DiversiLab typing. Automated DiversiLab typing system is a fast, easily operated, highly discriminatory and efficient genetyping
method. It can be recommended as an epidemiological analysis tool for foodborne illness. |
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| Keywords: | DiversiLab typing system Listeria monocytogenes rep-PCR epidemiological analysis |
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