| 亚临界水萃取及大孔树脂分离纯化制备紫草素 |
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| 引用本文： | 黄萍萍,杨日福,黄星歆,范晓丹,丘泰球.亚临界水萃取及大孔树脂分离纯化制备紫草素[J].食品科学,2010,31(8):1. |
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| 作者姓名： | 黄萍萍 杨日福 黄星歆 范晓丹 丘泰球 |
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| 作者单位： | 华南理工大学轻工与食品学院;华南理工大学物理科学与技术学院; |
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| 基金项目： | 国家自然科学基金项目(20776047); “十一五”国家科技支撑计划项目(2006BAD27B03) |
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| 摘 要： | 以新疆紫草为研究对象,考察亚临界水的动态循环和静态平衡两种萃取方式对紫草素提取率的影响。选取不同极性的大孔树脂和有机溶剂对提取液中的紫草素进行吸附、解析实验;使用高效液相-蒸发光散射(HPLC-ELSD)技术分析样品的纯度。结果显示:10MPa、120℃、1.39cm3/s的流量,动态循环萃取8min,紫草素提取率最高是0.216%;静态平衡萃取16min,紫草素提取率最高是0.198%。DA-201大孔树脂对紫草素的吸附效果最佳,恒温摇床振荡吸附24h,吸附率是89.7%;丙酮的解析效果最好,能够将93.2%的紫草素解析出来,且速率较快。经大孔树脂吸附分离后,紫草素的纯度由27.31%提高至85.07%。实验表明,亚临界水萃取、大孔树脂吸附分离能够有效制备纯度较高的紫草素。
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| 关 键 词： | 亚临界水 动态循环 静态平衡 紫草素 大孔树脂 |
Sub-critical Water Extraction and Purification by Macroporous Resin Adsorption of Shikonin from Arnebia euchroma |
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| HUANG Ping-ping,YANG Ri-fu,HUANG Xing-xin,FAN Xiao-dan,QIU Tai-qiu.Sub-critical Water Extraction and Purification by Macroporous Resin Adsorption of Shikonin from Arnebia euchroma[J].Food Science,2010,31(8):1. |
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| Authors: | HUANG Ping-ping YANG Ri-fu HUANG Xing-xin FAN Xiao-dan QIU Tai-qiu |
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| Affiliation: | HUANG Ping-ping1,YANG Ri-fu2,HUANG Xing-xin1,FAN Xiao-dan1,QIU Tai-qiu1,(1.College of Light Industry , Food Sciences,South China University of Technology,Guangzhou 510640,China,2.School of Physics,China) |
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| Abstract: | To prepare high-purity shikonin from Arnebia euchroma grown in Xinjiang autonomous region,subcritical water extraction and macroporous resin adsorption for purification were conducted.Effects of two extraction modes,namely dynamic circulation and static equilibrium,on shikonin yield were examined.Shikonin adsorption/desorption experiments were carried out using different polar macroporous resins and organic solvents.HPLC-ELSD was used to analyze the purity of the product obtained.Results showed that a maxim... |
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| Keywords: | sub-critical water dynamic circulation static equilibrium shikonin macroporous resin |
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