米曲霉Aspergillus oryzae As-W.6对脱氧雪腐镰刀菌烯醇的降解效果

研究米曲霉Aspergillus oryzae As-W.6对脱氧雪腐镰刀菌烯醇（deoxynivalenol，DON）的降解作用。经高效液相色谱（high performance liquid chromatography，HPLC）检测，不同初始质量浓度的DON经菌株As-W.6发酵7 d后，降解率均在35%以上；发酵14 d后，降解率均在90%以上；同时该菌株中提取的降解酶对不同初始质量浓度DON的降解率均达70%以上。对降解产物采用高效液相色谱-质谱（HPLC-mass spectrometry，HPLC-MS）分析检测，结果显示DON经该降解菌降解后，相对分子质量从原来的295.1变为281.2，减少了13.9，说明DON确实被该菌降解成为另一种物质。

Degradation Efficiency of Deoxynivalenol by Aspergillus oryzae As-W.6

This research work was conducted to investigate the degradation of deoxynivalenol (DON) by Aspergillus oryzae
As-W.6, which was isolated and preserved in our laboratory. Results showed the degradation rate of DON at all initial
concentrations investigated was greater than 35% after 7 d fermentation by strain As-W.6 and reached higher than 90%
with fermentation time up to 14 d. Moreover, the strain could degrade more than 70% of DON with the enzyme produced
by it. HPLC-MS analysis revealed that the relative molecular mass of DON was decreased from 295.1 to 281.2 after the
degradation, a decrease of 13.9, suggesting that DON was degraded into another substance by As-W.6.