| 产胞外多糖植物乳杆菌的筛选及粗多糖的活性研究 |
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| 引用本文： | 冯小婉,夏永军,王光强,艾连中.产胞外多糖植物乳杆菌的筛选及粗多糖的活性研究[J].食品科学,2016,37(13):125-129. |
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| 作者姓名： | 冯小婉 夏永军 王光强 艾连中 |
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| 作者单位： | 上海理工大学医疗器械与食品学院，上海 200093 |
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| 基金项目： | 国家自然科学基金面上项目（31371809）；上海科委国际科技合作项目（14390711700） |
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| 摘 要： | 通过观察乳酸菌菌落拉丝状况并测定其胞外多糖(exopolysaccharide,EPS)的产量,筛选出1株所产EPS黏性好、产量高的乳酸菌AR307,经鉴定为植物乳杆菌。为得到更多的胞外多糖,对植物乳杆菌AR307的发酵条件进行优化,确定其在发酵温度32℃、发酵时间20 h条件下的产糖量为389 mg/L。在体外实验中,所得胞外多糖具有抑制HT-29肿瘤细胞活性、降低血糖水平的作用。
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| 关 键 词： | 植物乳杆菌 发酵条件 胞外多糖 |
Screening of Exopolysaccharide (EPS)-Producing Lactobacillus plantarum and Bioactivity of the EPS |
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| FENG Xiaowan,XIA Yongjun,WANG Guangqiang,AI Lianzhong.Screening of Exopolysaccharide (EPS)-Producing Lactobacillus plantarum and Bioactivity of the EPS[J].Food Science,2016,37(13):125-129. |
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| Authors: | FENG Xiaowan XIA Yongjun WANG Guangqiang AI Lianzhong |
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| Affiliation: | School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China |
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| Abstract: | The present study describes the screening of lactic acid bacterial (LAB) strains with high exopolysaccharide
(EPS)-producing ability by observing whether they could form sticky colonies. One strain named AR307 was finally
obtained and identified as Lactobacillus plantarum. In order to enhance EPS yield, the fermentation conditions for producing
EPS by Lactobacillus plantarum AR307 were optimized. The yield of EPS produced by AR307 reached up to 389 mg/L
after 20 h fermentation at 32 ℃. In vitro assay showed that the EPS had good inhibitory activity against colon cancer HT-29
cells and possessed an antidiabetic effect. |
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| Keywords: | Lactobacillus plantarum fermentation conditions exopolysaccharide (EPS) |
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