湖北掌叶覆盆子叶降血脂作用的动物实验和人群研究

目的:探讨湖北掌叶覆盆子叶对高脂模型动物和高血脂人群的降血脂作用。方法:采用预防性大鼠脂质代谢紊乱模型,50只大鼠根据血清总胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白胆固醇(HDL-C)水平随机分成对照组、高脂模型组和掌叶覆盆子叶0.5、1.0、2.0g/kgbw剂量组,对照组饲喂基础饲粮,高脂模型组和掌叶覆盆子叶组喂饲高脂饲粮,掌叶覆盆子叶各剂量组灌胃给予水提取物,对照组和模型组给予蒸馏水,连续30d后,取血,分离血清,检测TC、TG和HDL-C水平。选择112高血脂症人群,随机盲法按血脂水平、年龄、性别、饮食等因素均衡分为试验组和对照组,试验组饮用掌叶覆盆子叶袋泡茶每日3次,每次2g,对照组不给予任何干预措施,不改变日常生活和饮食习惯,连续30d,取血,分离血清,检测TC、TG和HDL-C水平。结果:动物实验中,覆盆子叶1.0、2.0g/kgbw剂量组大鼠血清TC、TG显著低于高脂模型组(p<0.05)。人群试验中,试验组试验结束时的TC、TG与试验前以及对照比较,差异均有显著性(p<0.05);试验组降血脂的总有效率为80.4%。结论:湖北掌叶覆盆子叶有显著的降血脂作用。

Effects of Hubei Rubus chingii Hu Leaf on Serum Lipid Metabolism in Hyperlipidemia Rats and Human Adults

Objective: To study the effect of Hubei Rubus chingii Hu leaf on serum lipid metabolism in hyperlipidemia rats and adults. Methods: In hyperlipidemia animal models, according to the serum TC, TG and HDL-C concentrations levels, 50 rats were randomly divided into five groups: control group, hyperlipidemia model group, and 0.5, 1.0, 2.0 g/kg bw dose groups. Control group was fed with basic feed and other groups were fed with high percent lipid feed. Dose groups were ingested with Rubus chingii Hu water soluble extract for 30 days, and control and model groups ingested with water, then the serum TC, TG and HDL- C concentrations were determined. In human intervention trials, 112 hyperlipidemia adult volunteers were selected and ran- domly divided into two groups, namely experiment group and control group, according to their serum TC, TG levels, age, gender and diet etc. Experiment adults group were given Rubus chingii Hu leaf drinking in the dose of 3 times per day and 2g per time without changing daily custom and diet, while control group adults were not given any intervention trematmeat. 30 days later, serum TC, TG and HDL-C levels were determined. Results: In animal study, compared with control, serum TC, TG levels of 1.0, 2.0 g/kg bw dose of Rubus chingii Hu leaf groups decrease significantly (p<0.05) after 30 days of treatments. At the end of human trial, comparison with control, serum TC, TG levels of experiment groups decrease significantly(p<0.05). whereas the rate of effective lowering lipid level is 80.4% in experiment group at the end of trials. Conclusion: Hubei Rabus chingii Hu leaves show significant effects of decceasing regulating serum lipid levels in rats and human adults as well.