红檵木花色素TLC分离与定性研究

通过对红檵木花色素的硅胶H和硅胶G薄板层析（TLC）分离研究，得到其TLC最佳层层剂（体积比）是：水：乙醇：正丁醇：乙酸乙酯=12：8：24：36。以最佳展层剂展开花中的色素，得到颜色及相应R＋值完全一致的7个色斑，反映该花色素含七种色素单体成分。对色素母液和各色素单体成分样液的200-800nm扫描测定表明，色素母液的紫外特征吸收峰是277nm，各单体成分的特征吸吸收峰分别为：272、278、277、270、273、274、271nm，推论认为277nm单体成分（成分③）在花中含量最大。结合色素母液及色素单体样液的甲醇和乙醇样液扫描分析与花青素、黄酮类及蒽醌类特征显色反应研究结果，基本确认红檵木花中的色素主要成分属二氢黄酮醇类。

Study on the Separating by TLC and Properties Defining of the Pigments from Loropetalum chinense var.rubrum flower

Seperating by silca-G&H thin-layer chromatography(TLC) on the pigments from Loropetalum chinense var.rubrum flower, the optimum volume ratio of the seperating layer reagent is water12 to eathnol 8 to plus-butanol 24 to acetic ester36. To developing the flower pigment by the optimum layer reagent, seven colour spot, which obtained from the pigment , have the same colour and the same Rf. Seperating and scanning results showed that the characteristic wavelength of the pigment in the L. chinense var.rubrum flower is 277nm and it,s seven mono-component are 272, 278, 277, 270, 273, 274, 271nm repectively, and the maximum content of the mono-component is the third component (277nm). Combinding the results of scanning on the pigment component with the chracteristic colour reaction in Anthocyanidin and Flavonoid and Anthraquinone of the pigment, the main mono-component in the flower pigment must belongs to flavanonol.