从香菇中浸提香菇多糖的方法对比研究

本实验以干香菇粉末为原料,以蒽酮-硫酸法作为香菇多糖的测定方法,通过正交试验综合分析香菇多糖在热水浸提法、复合酶法、超声波辅助法浸提工艺下的多糖得率,比较三种工艺的优缺点。实验结果表明,在最佳工艺条件下,复合酶法多糖得率为7.530%,热水浸提法为3.229%,超声波辅助法为4.669%。故复合酶法是香菇多糖浸提工艺的首选,其最佳工艺条件是纤维素酶含量0.1%、果胶酶0.5%、木瓜蛋白酶2.0%、提取时间80min,多糖得率为7.530%。

Study on Extraction of Lentinan from Dried Lentinus edodes with Different Methods

In this study the content lentinan from dried Lentinus edodes was measured by the anshtone-sulfutic method.Through orthogonal test,the extraction ratios of lentinan by hot water method,multi-enzymatic method,ultrasonic wave-assisted method were respectively analyzed,and what was more,the advantages and disadvantages of three extraction methods were compared.The results showed that under the optimal conditions,the extraction ratios of lentinan are 7.530% in multi-enzymatic process,3.229% in hot water process,and 4.669% in ultrasonic wave-assisted process.Therefore the best extraction process of lentinan is multi-enzymatic method,and its optimal conditions are 0.1% cellulase,0.5% pectinase,2.0% papain,and extraction temperature 80 min.