| 产聚半乳糖醛酸酶的黑曲霉诱变育种 |
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| 引用本文： | 张媛媛,郝 林,王 倩.产聚半乳糖醛酸酶的黑曲霉诱变育种[J].食品科学,2013,34(7):231-233. |
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| 作者姓名： | 张媛媛 郝 林 王 倩 |
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| 作者单位： | 山西农业大学食品科学与工程学院，山西 太谷 030801 |
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| 摘 要： | 利用透明圈法对黑曲霉SH312菌株进行初筛，得到黑曲霉SH312-26菌株。对其进行紫外线诱变，筛选出SH312-26-19菌株，该菌株聚半乳糖醛酸酶酶活力为113.68kU/mL，较出发菌株SH-312-26提高了1.44倍；果胶酯酶酶活力为14.34kU/mL，较出发菌株提高了0.14倍，其幅度远小于聚半乳糖醛酸酶酶活的提高幅度。突变株SH312-26-19经斜面传代培养了5代，产酶遗传特性稳定。
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| 关 键 词： | 聚半乳糖醛酸酶 诱变育种 筛选 |
| 收稿时间： | 2011-12-24 |
Mutation Breeding of a High Polygalacturonase-Producing Aspergillus niger Strain |
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| ZHANG Yuan-yuan,HAO Lin,WANG Qian.Mutation Breeding of a High Polygalacturonase-Producing Aspergillus niger Strain[J].Food Science,2013,34(7):231-233. |
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| Authors: | ZHANG Yuan-yuan HAO Lin WANG Qian |
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| Affiliation: | College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China |
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| Abstract: | Aspergillus niger strain SH312-26 was isolated by apparent circle method from strain SH312. A mutant strain
named as SH312-26-19 was obtained by UV radiation using SH312-26 the original strain. The polygalacturonase activity
of SH312-26-19 was 113.68 kU/mL, a 1.44-fold increase than that of strain SH-312-26. The pectin esterase activity was
14.34 kU/mL, a 0.14-fold increase than that of the original strain. After 5 passages on slant culture medium, the mutant
remained genetically stable. |
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| Keywords: | polygalacturonase mutation breeding selection |
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