基于斑马鱼模型研究不同磷脂促血管生成活性

目的：利用斑马鱼模型研究不同来源和不同类型的磷脂对血管生成的促进作用及其活性差异。方法：将受精后发育24 h和72 h的斑马鱼胚胎脱膜，分别用于评估各磷脂样品（分别以大豆、蛋黄和南美白对虾虾头制备）对斑马鱼体节间血管（intersegmental vessel，ISV）和肠下静脉（subintestinal vein vessel，SIV）生长情况的影响，设置空白对照组、模型组（0.25 μg/mL PTK787）、阳性对照组和磷脂样品组（20、40、80 mg/mL），给药24 h后用荧光显微镜观察并记录各实验组转基因斑马鱼ISV和SIV的生长情况。结果：对于ISV受损斑马鱼而言，3 种磷脂提取物以及2 种磷脂市售产品（药品和保健品）均表现出明显的血管生成促进作用，对于SIV而言，3 种磷脂提取物以及4 种磷脂市售产品（药品和保健品）均表现出明显的血管生成促进作用，相对而言，南美白对虾虾头磷脂促进血管生成活性更强，效果更加明显；5 种磷脂标准品表现出不同程度的血管生成促进作用，对于ISV受损斑马鱼而言，磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰丝氨酸以及高质量浓度（80 mg/mL）的鞘磷脂表现出明显的促血管生成作用，对于SIV受损斑马鱼而言，所有的磷脂标准品均表现出较明显的血管生成促进作用。结论：不同质量浓度的磷脂提取物、市售产品以及磷脂标准品对斑马鱼均具有一定的促血管生成活性。

Angiogenic Promoting Activity of Different Phospholipids in Zebrafish

Purpose: The pro-angiogenic effects of different sources and types of phospholipids in zebrafish were evaluated and compared. Methods: Zebrafish embryos at 24 and 72 h post fertilization (hpf) were used to evaluate the effects of phospholipid samples (prepared from soybean, egg yolk or white shrimp head) on the growth of intersegmental vessel (ISV) and subintestinal vein vessel (SIV). Six experimental groups were set up, including blank control, model (treated with 0.25 μg/mL PTK787), positive control and phospholipid treatment (at concentrations of 20, 40 and 80 μg/mL). After incubation for 24 h, the ISV and SIV growth of transgenic zebrafish in each experimental group was observed and recorded by fluorescence microscopy. Results: The three phospholipids prepared in this study and two commercially available phospholipid products (iodized lecithin tablets and polyene phosphatidylcholine capsules) showed obvious pro-angiogenic activity on zebrafish with damaged ISV. All three phospholipids and four commercially available phospholipid products (iodized lecithin tablets, polyene phosphatidylcholine capsules, soybean phospholipid soft capsules, and fish oil capsules) showed obvious pro-angiogenic activity on SIV. Among these drugs, phospholipids in the head of Penaeus vannaceus had the strongest pro-angiogenic activity. Five phospholipid standards showed different angiogenesis promoting effects. Phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS) and high concentration (80 μg/mL) of sphingomyelin (SM) showed obvious pro-angiogenesis on ISV. All phospholipid standards showed obvious pro-angiogenic effects on SIV. Conclusion: Different concentrations of phospholipid extracts, commercially available phospholipid products and phospholipid standards had pro-angiogenic activity on zebrafish.