| 荧光衍生化法测定新疆特色葡萄干中总亚硫酸盐含量 |
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| 引用本文： | 王金霞,林江丽,鹿毅,王吉德.荧光衍生化法测定新疆特色葡萄干中总亚硫酸盐含量[J].食品科学,2016,37(4):212-215. |
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| 作者姓名： | 王金霞 林江丽 鹿毅 王吉德 |
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| 作者单位： | 1.新疆大学化学化工学院，石油天然气精细化工教育部和新疆维吾尔自治区共建重点实验室，新疆 乌鲁木齐 830046；;
2.新疆产品质量监督检验研究院，新疆 乌鲁木齐 830004 |
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| 基金项目： | 新疆维吾尔自治区自然科学基金项目(2013211A015) |
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| 摘 要： | 建立荧光衍生化法快速、准确测定新疆特色葡萄干中总亚硫酸盐的分析方法。用N-(9-吖啶基)马来酰亚胺为衍生化试剂,使其与葡萄干中的亚硫酸盐发生衍生化反应产生具有强荧光的衍生化产物,采用荧光光谱仪对其进行分析检测。结果表明,在1.0~43.2 mg/L质量浓度范围内相关系数(R~2)为0.999 3,方法的检出限为0.50 mg/L,3个添加水平时,平均回收率为92.2%~100.2%,相对标准偏差(n=3)不大于3.80%。利用该法对葡萄干中总亚硫酸盐的测定结果与国标法测得结果一致,说明该方法可快速准确地对新疆特色葡萄干中总亚硫酸盐进行测定,为干果中亚硫酸盐残留量的测定提供参考。
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| 关 键 词： | 葡萄干 总亚硫酸盐 荧光衍生化法 |
Determination of Total Sulfite in Xinjiang Specialty Raisin by Fluorescence Derivatization |
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| WANG Jinxia;LIN Jiangli;LU Yi;WANG Jide.Determination of Total Sulfite in Xinjiang Specialty Raisin by Fluorescence Derivatization[J].Food Science,2016,37(4):212-215. |
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| Authors: | WANG Jinxia;LIN Jiangli;LU Yi;WANG Jide |
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| Affiliation: | 1. Key Laboratory of Oil and Gas Fine Chemicals Ministry of Education and Xinjiang Uyghur Autonomous Region,;
College of Chemistry and Chemical Engineering, Xinjiang University, Ürümqi 830046, China;
2. Xinjiang Supervision and Inspection Institute for Product Quality, Ürümqi 830004, China |
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| Abstract: | A rapid and accurate method for the determination of total sulfite in Xinjiang specialty raisin was developed using
fluorescence derivatization. N-(9-Acridinyl) maleimide (NAM) reacted with the sulfite in raisin to form strong fluorescent
derivatives, which were then detected by fluorometry. The linear range of the method was between 1.0 and 43.2 mg/L, with a
correlation coefficient (R2) of 0.999 3. At three spiked levels, the average recoveries of sulfite in raisin ranged from 92.2% to
100.2% with relative standard deviations lower than 3.80% (n = 3). Furthermore, the results obtained by this method for the
total sulfite content of raisin were consistent with those obtained by the pararosaniline method. These results indicate that the
fluorescence derivatization method can accurately and rapidly determine total sulfite in Xinjiang specialty raisin. |
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| Keywords: | raisin total sulfite fluorescence derivatization |
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