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玉米须总黄酮的提取与抗氧化活性研究
引用本文:方 敏,占才贵,宫智勇.玉米须总黄酮的提取与抗氧化活性研究[J].食品科学,2009,30(18):206-208.
作者姓名:方 敏  占才贵  宫智勇
作者单位:武汉工业学院食品科学与工程学院
摘    要:以乙醇体积分数、浸提时间、固液比、提取温度为考察因素对玉米须总黄酮的提取条件进行正交设计试验,并用DPPH 和FRAP 两种方法测定了总黄酮的抗氧化活性。结果表明:玉米须总黄酮提取的最佳工艺条件为乙醇体积分数40%、提取时间2h、固液比1:25(m/V)、提取温度80℃,此时玉米须总黄酮的提取率为1.67%;玉米须总黄酮的抗氧化作用明显,对DPPH 自由基的清除率达到77.18%,总抗氧化能力FRAP 值为691.04μmol/L。

关 键 词:玉米须  总黄酮  提取  抗氧化  
收稿时间:2009-06-20

Extraction and Antioxidant Activities of Total Flavonoids from Corn Silk
FANG Min,ZHAN Cai-gui,GONG Zhi-yong.Extraction and Antioxidant Activities of Total Flavonoids from Corn Silk[J].Food Science,2009,30(18):206-208.
Authors:FANG Min  ZHAN Cai-gui  GONG Zhi-yong
Affiliation:College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
Abstract:Total flavonoids in corn silk were extracted with ethanol and analyzed for their DPPH scavenging activity and total antioxidant capacity. The effects of ethanol concentration, extraction time, material/liquid ratio and extraction temperature were evaluated for optimizing the extraction procedure of total flavonoids. A maximum yield of total flavonoids of 1.67% was obtained by the 2 h extraction using 40% ethanol at 80 ℃. The total flavonoids from corn silk had marked antioxidant activities: the scavenging rate against DPPH free radicals was as high as 77.18% and the FRAP value reached 691.04 μmol/L.
Keywords:corn silk  total flavonoids  extraction  antioxidant activities  
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