离子色谱直接电导法检测糖蜜中的甜菜碱

建立甜菜碱的离子色谱直接电导测定法,用于糖蜜中提取甜菜碱的生产工艺。采用WY阳离子交换柱(150mm×4.6mm),柱温30℃,2.0mmol/L甲烷磺酸溶液为流动相,流速1.0mL/min,电导检测池。结果表明,甜菜碱在0.5～50mg/L质量浓度范围内线性关系良好(r=0.9995),加标回收率在93.5%～99.7%之间,检测限为0.15mg/L。所建方法适用于糖蜜中提取甜菜碱生产工艺中质量控制。

Determination of Betaine in Molasses by Ion Chromatography with Direct Conductivity Detection

An ion chromatography-direct conductivity detection method was developed for determining betaine in molasses.The chromatographic separation of betaine was achieved on a WY cation column(150 mm × 4.6 mm i.d.) using 2.0 mmol/L MSA as mobile phase at a flow rate of 1.0 mL/min.The column was set at 30 ℃.Betaine was detected using a direct conductivity detector.A good linear range of the calibration curve was achieved over the concentration of 0.5-50 mg/L(r = 0.9995) with recovery rate of 93.5%-99.7% and detection limit of 0.15 mg/L.The method was suitable for the determination of betaine and quality control for betaine extraction from molasses.