| 竹叶黄酮纯化中吸附剂的优选及解析特性研究 |
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| 引用本文： | 游辉,孙爱东,唐玲,潘娜,陈健.竹叶黄酮纯化中吸附剂的优选及解析特性研究[J].食品科学,2010,31(8):24. |
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| 作者姓名： | 游辉 孙爱东 唐玲 潘娜 陈健 |
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| 作者单位： | 北京林业大学生物科学与技术学院食品系; |
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| 基金项目： | “十一五”国家科技支撑计划项目(2006BAD19B08;2008BADA9B04) |
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| 摘 要： | 选择7种大孔吸附树脂,通过研究其对竹叶黄酮的吸附率和解吸率,筛选出较优的竹叶黄酮吸附剂。结果表明:AB-8型大孔树脂吸附量大,易于洗脱,纯化分离效果好;获得竹叶黄酮最佳分离纯化工艺参数为上柱溶液pH5.0,以1.0mL/min的吸附流速上样,用4倍床体积的60%乙醇溶液以1.5mL/min洗脱速率洗脱。该工艺生产的竹叶黄酮纯度达到54.16%。
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| 关 键 词： | 大孔吸附树脂 竹叶 黄酮 分离 纯化 |
Macroporous Resin Adsorption for Purification of Crude Flavonoids from Bamboo Leaves |
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| YOU Hui,SUN Ai-dong,TANG Ling,PAN Na,CHEN Jian.Macroporous Resin Adsorption for Purification of Crude Flavonoids from Bamboo Leaves[J].Food Science,2010,31(8):24. |
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| Authors: | YOU Hui SUN Ai-dong TANG Ling PAN Na CHEN Jian |
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| Affiliation: | YOU Hui,SUN Ai-dong,TANG Ling,PAN Na,CHEN Jian(Department of Food Science,College of Biological Sciences , Biotechnology,Beijing Forestry University,Bejing 100083,China) |
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| Abstract: | To screen an optimal adsorbent for purification of flavonoids from bamboo leaves,7 macroporous resins were compared for difference in adsorption and desorption performance.AB-8 resin was chosen due to the merits of large adsorption capacity,easy desorption and high resolution.Besides,the optimal adsorption/desorption process for purification of bamboo leaf flavonoids using AB-8 resin was investigated.Results showed that the optimal pH and flow rate of sample solution were 5.0 and 1 mL/min,respectively and t... |
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| Keywords: | macroporous resins bamboo leaves flavonoids separation purification |
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