刺梨总黄酮的含量测定及资源利用

目的：测定刺梨中总黄酮的含量，为进一步开发利用贵州丰富的刺梨资源提供科学依据。方法：正交设计优选刺梨中总黄酮的提取条件，用芦丁作对照品，三氯化铝作显色剂，测定波长在420nm，比色法测定刺梨中总黄酮含量。结果：优选提取条件是：用65％乙醇提取4h，每次提取体积100ml，提取一次。测定刺梨中总黄酮的含量1，2，3号样分别为0．773％，0．874％，0．893％。结论：优选提取方法效率高，含量测定方法操作简便，准确，稳定性好。

Determination of Content of Total Flavonoids in Cili and Resources Development Prospect

Object:To provide scientific basis for further developing and using ric h Cili resources in Guizhou.Methods:The content of total flavon oids was measured,using rutin as con trol and aluminumtrichloride as colori-metricreagentandcolorimetricass ayat420nm.Result:Theoptimumextr actionconditions were:toextract4hwith65%C 2 H 5 OH?with extract volume 100ml everytime .The content of total flavonoid of1?2and 3were 0.773%?0.874%and0.893%respectively.Con dition:Theoptimumextractionmeth odwas efficient.Thedeteminationo f the flavonoid content was simple,steady,accurate and reproducible.