5种长白山野生食用菌的抗肿瘤活性研究

为研究长白山野生食用菌的抗肿瘤活性,选取灵芝、桦褐孔菌、桑黄、猴头蘑、香菇5种长白山野生食用菌为试验材料,分别进行体外、体内抗肿瘤试验。体外抗肿瘤试验采用MTT法研究野生食用菌提取物对肿瘤细胞的抑制作用;体内试验测定对小鼠S-180肉瘤细胞株的生长抑制率。结果显示:体外试验中,在最高浓度1mg/mL时,灵芝对HeLa及HepG2两种细胞株的抑制效果最好,桑黄对MCF-7细胞株的抑制率最高,而猴头菇对A549细胞显示了最强的抑制效果。并且除灵芝外其余4种食用菌的抑制作用与药物浓度存在依赖关系。体内试验中,5种食用菌对小鼠S-180肉瘤细胞株均显示了明显的抑瘤效果,其中猴头蘑高剂量组达到最大值,为75.92%,且香菇提取物在低剂量时就已显示了很高的肿瘤抑制率(为69.39%)。

Study on antitumor activities of five kinds of wild edible mushrooms in Changbai Mountain

To study the anti-tumor activities of wild edible mushrooms of the Changbai Mountain, the experiment selected Ganoderma Lucidum, Inonotus Obliquus, Phellinus, Hericium Erinaceus, Lentinula Edodes five kinds of Changbai Mountain wild edible mushrooms as materials, the experiment was divided into in vitro and in vivo study. The inhibition effect of tumor cells was tested by MTT method, and the inhibition ratio of mouse sarcoma S-180 was calculated in vivo study. The results showed that Ganoderma Lucidum has the highest inhibition ratio on the HeLa and HepG2 cell lines. Phellinus has the highest inhibition rate on MCF-7 cell line, Hericium Erinaceus showed the strongest inhibitory effect on the A549 cell lines in vitro study. And the other kinds of wild edible mushrooms displayed obviously dose-dependent inhibitory effect but except Ganoderma Lucidum. In vivo study, all the wild edible mushrooms showed the tumor suppression effect in mouse sarcoma S-180 cell line, which the maximum, high dose group of Hericium Erinaceus was 75.92%, but the lowest doses of Lentinula Edodes extract has showed high tumor inhibition rate (69.39%).