不同物种茶油脂肪酸组成及其在Sn-2位上的分布

试验以十五烷酸为内标,定量分析9种不同物种茶油的脂肪酸组成;用胰脂肪酶专一性地水解茶油甘油三酯中Sn-1和Sn-3位上的脂肪酸,并经薄层层析分离出Sn-2位甘一酯,采用气相色谱仪对Sn-2位脂肪酸组成进行测定。结果表明:9个不同物种茶油主要由棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸5种脂肪酸组成,浙江红花油茶的油酸和不饱和脂肪酸的含量最高,分别为82.79g/100g和84.77g/100g;且脂肪酸之间存在一定的相关性;Sn-2位上的脂肪酸主要为油酸和亚油酸,短柱茶在Sn-2位的油酸和不饱和脂肪酸含量最高,分别为82.96%,92.89%。表明茶油是一种不饱和脂肪酸含量高且容易被人体消化吸收的油脂。

Analysis of fatty acids component and distribution in Sn-2 in oils from different Camellia spp.

The contents of fatty acids in nine different oils of Camellia spp.wereanalyzed by GC using methyl pentadecanoate as the internal standard. The triglycerides were specially hydrolyzed by the use of pancreatic lipase, 2-monoglyceride was isolated by thin-layer chromatography and then was analyzedby gas chromatography. The results showed that five kinds of fatty acids presented in tested samples of which were palmitic acid, stearic acid, oleic acid, linoleic aicd and linolenic acid. Contents of oleic acid and unsaturated fatty acids in Camellia chekiangoleosawere the highest with 82.79 and 84.77 g/100 g, respectively. The fatty acids in Sn-2 were mainly oleic acid and linoleic acid, and of which were the highest in C. brevistylaoil with 82.96% and 92.89%,respectively. It means that the major fatty acids present in camellia seed oil were the unsaturated fatty acidswhich are easily digested.