超声协同糖基化对蛋清粉致敏性及结构的影响

以市售蛋清粉为研究对象,通过电泳、紫外光谱、荧光光谱和酶联免疫吸附等方法研究超声波协同糖基化修饰对其蛋白结构和致敏性的影响。结果表明,超声波协同糖基化修饰能显著降低蛋清粉的致敏性,致敏性随超声强度的增加呈先增加后降低的趋势,且在15 min、600 W的条件下有最大程度降低。这和其结构变化密切相关,经复合处理后,蛋清蛋白分子量显著增加,同时三级结构发生显著变化,使表面疏水性增强、内源荧光和自由氨基含量降低,导致其致敏性降低。超声波协同糖基化修饰是一种良好的降低蛋清粉致敏性的方法。

Effect of ultrasounds ynergistic glycosylation on the sensitization and the structure of egg white powder

The commercially available egg white powder was taken as the research object. The effects of ultrasound synergistic glycosylation on protein structure and sensitization were studied by electrophoresis, ultraviolet spectrum, fluorescence spectroscopy and ELISA. The results showed that ultrasound combined with glycosylation could reduce the sensitization of egg white powder significantly, and the reduce of hypersensitivity level was the largest when the ultrasound intensity was around 15 min and 600 W. This change was closely related to its structure, and the egg white protein molecular weight increased significantly after combined treatment. Moreover, the three stages of structure change significantly resulted in the increase of surface hydrophobicity, and the decrease of endogenous fluorescence and free amino groups. These structural changes led to the decrease of their sensitization.The results showed that ultrasound combined with glycosylation was a good way to reduce the sensitization of egg white powder, and this provided a more practical basis and theoretical guidance for the development of desensitized egg products.