玫瑰红景天醇提物不同溶剂萃取物的生物活性

以无水乙醇为溶剂,用索氏提取器对玫瑰红景天粉末进行回流提取,回收乙醇后将乙醇提取物分散和溶解在水相中,依次用环己烷、氯仿、乙酸乙酯、正丁醇分别萃取,得到不同溶剂萃取物。用比色法测定不同溶剂萃取物和水相物中总酚和总黄酮含量,DPPH·法、Fenton法、普鲁士蓝法评价其抗氧化活性以及测试最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明,乙酸乙酯萃取物中总酚和总黄酮含量最高,分别为(469.32±1.15),(338.57±0.92)mg/g萃取物;不同萃取物和水相物对DPPH·、·OH有不同程度的清除作用且有还原能力,其中乙酸乙酯萃取物和正丁醇萃取物有相对较好的抗氧化活性;总酚和总黄酮含量与抗氧化活性呈显著相关性(P<0.05);对选用的9种菌株均有抑制和灭活作用,MBC值在0.28~16.94mg/mL,对金黄色葡萄球菌株灭活作用更为显著,其中氯仿萃取物和乙酸乙酯萃取物的抗菌效果相对较好。玫瑰红景天乙醇提取物的不同溶剂萃取物及水相物均有抗氧化活性和抗菌活性,有潜在的应用价值。

Bioactivities of different solvents extracts from the ethanol extract of Rhodiola rosea

The ethanol extract was obtained from the powder of Rhodiola rosea with ethanol recycling in Soxhlet extractor,and was dissolved in water to disperse system after recovering the ethanol,then respectively extracted with cyclohexane,chloroform,ethyl acetate and butanol to get different solvent extracts.These different solvent extracts were determined total phenols and flavonoids content by a spectrophotometer,in the same time,their anti-oxidation activities were tested by DPPH·method,Fenton reaction method and prussian blue method with using Vc as a reference substance,and their antibacterial activities were evaluated by using minimum inhibitory concentration and minimum bactericidal concentration.The results showed that the highest content of total phenols and flavonoids were in ethyl acetate extract,its content was(469.32±1.15),(338.57±0.92)mg/g extract,these different solvent extracts and the aqueous extract had obvious action to scavenge effect for DPPH·and·OH,showed a strong total reduction capacity,its ethyl acetate extract and butanol extract exhibited relatively good antioxidation activities,the content of total phenols and flavonoids related to the antioxidant activity(P<0.05),the MBC values of these extracts between 0.28~16.94 mg/mL showed inhibitory activities against the nine bacteria strains,inhibited the growth of testing bacteria and sterilized them especially against Staphylococcus aureus,its chloroform extract and ethyl acetate extract were very obvious to antibacterial effect.These different solvent extracts and the aqueous extract of the ethanol extracts from Rhodiola rosea showed certain antioxidant activities and antibacterial activities,they had potential applications.