| 基于核酸适配体调控噻菁染料聚集体构建铅离子检测方法 |
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| 引用本文： | 赵晗,于丽佳,吴志生,张颖琳,张华伟,师晓栋.基于核酸适配体调控噻菁染料聚集体构建铅离子检测方法[J].食品与机械,2021,37(1):73-78. |
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| 作者姓名： | 赵晗 于丽佳 吴志生 张颖琳 张华伟 师晓栋 |
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| 作者单位： | 国家卫生健康委职业安全卫生研究中心;泰州医药高新技术产业开发区管理委员会 |
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| 基金项目： | 国家自然科学基金(批准号:21907021);国家卫生健康委职业安全卫生研究中心重点资助项目(编号:2020017,2020020,2020021)。 |
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| 摘 要： | 基于调控噻菁染料(Dye2)的超分子自组装性质及其与核酸适配体(T30695)特异性结合的能力,构建了Pb2+的特异性识别模块。结果表明,当体系中加入Pb2+时,T30695单链形成G-四链体,并将二聚体形式存在的噻菁染料诱导解聚为单体,引起422,445nm处紫外吸收峰变化,仅需通过UV-vis光谱仪便可实现对Pb2+的高选择性、高灵敏度识别。该体系在Pb2+浓度为0.25~10.00μmol/L时具有线性关系(R2=0.992 7),其检出限为0.115μmol/L。
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| 关 键 词： | 核酸适配体 噻菁染料 铅离子(Ⅱ) G-四链体 检测 |
| 收稿时间： | 2020/6/9 0:00:00 |
Construction of lead (II) ion detection method based on regulation of thiacyanine self-assembly by nucleic acid aptamer |
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| ZHAOHan,YULiji,WUZhisheng,ZHANGYinglin,ZHANGHuawei,SHIXiaodong.Construction of lead (II) ion detection method based on regulation of thiacyanine self-assembly by nucleic acid aptamer[J].Food and Machinery,2021,37(1):73-78. |
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| Authors: | ZHAOHan YULiji WUZhisheng ZHANGYinglin ZHANGHuawei SHIXiaodong |
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| Affiliation: | (National Center for Occupational Safety and Health,NHC,Beijing 102308,China;Taizhou Medical New&Hi-tech Industrial Development Zone,Taizhou,Jiangsu 225300,China) |
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| Abstract: | In this paper,regulation of self-assembly properties of thiacyanine dye(Dye2)by nucleic acid aptamer(T30695)was used to construct a specific recognition unit for detecting Pb2+.G-quadruplexes were formed to interact with dye 2 monomers,which could disassemble dimers of Dye2 into monomer,in the presence of Pb2+.The absorption vales at 422 nm via 445 nm was used as a specific recognition signal to achieve the purpose of specificity and high sensitivity identification of Pb2+only by UV-vis spectra.This system has a good linear relationship from 0.25 to10.00μmol/L(R2= 0.992 7),and the detection limit is0.115μmol/L. |
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| Keywords: | nucleic acid aptamer thiacyanine dye lead ion(Ⅱ) G-quadruplexes detection |
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