嗜铅乳酸菌JT1传代培养稳定性研究

从表型特征和益生特性两方面研究嗜铅乳酸菌JT_1在优化后的M1培养基和加入魔芋粉的M2培养基中连续传代的稳定性。结果表明,嗜铅乳酸菌JT_1在两种培养基中,连续传至1 000代时,形态并没有发生改变,菌落呈不透明白色突起,表面光滑,与原始菌株的菌落形态一致;连续传代并没有改变培养周期内总生物量和周期末活菌数的变化;菌株JT_1对p H为2.5的人工胃液、p H为8.0的人工肠液以及0.3%的胆盐的耐受能力基本保持稳定;菌株所特有的吸附Pb2+功能在传代过程中没有受到很大的影响,吸附率基本稳定在60%左右。为嗜铅乳酸菌JT_1的可持续性生产提供了可靠依据。

Stability of lead-resistant lactic acid bacteria JT1 in subculture

The stability of the continuous subculture of lead-resistant lactic acid bacteria JT1 in the optimized medium (M1) and MRS medium added with konjac flour (M2) was researched in the aspects of the phenotypic characteristics and probiotic characteristics. The results showed that the form of lead-resistant lactic acid bacteria JT1 did not changed in both M1 and M2 after the 1000 generations. The modality of colonies was opaque white protuberance with smooth surface and consistent with the original isolated colony. Continuous culture didn''t change the total biomass in culture cycle and the living bacteria number at the end of the culture cycle. The tolerance of JT1 kept stable in artificial gastric juice(pH 2.5), artificial intestinal juice(pH 8.0) and 0.3% bile salts.In particular, the specific function of adsorption of Pb2+ was nearly not affected during the passage, and the adsorption rate was basically stable at around 60%. These results provide the reliable basis for the stainable production of lead-resistant lactic acid bacteria JT1.