| 谷氨酸发酵过程中N-乙酰-谷氨酰胺的积累 |
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| 引用本文： | 赵普亚,黄磊,孙村民,曾祥峰,贾士儒,孙际宾.谷氨酸发酵过程中N-乙酰-谷氨酰胺的积累[J].现代食品科技,2011(9):1057-1061. |
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| 作者姓名： | 赵普亚 黄磊 孙村民 曾祥峰 贾士儒 孙际宾 |
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| 作者单位： | 天津科技大学生物工程学院;中国科学院天津工业生物技术研究所系统微生物工程院重点实验室;山东省公安厅刑侦局; |
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| 基金项目： | 科技部973计划(2009CB724703); 中国科学院重要方向性项目(KSCX2-YW-G-030) |
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| 摘 要： | 在利用HPLC分析谷氨酸发酵过程样品时,发现在色谱峰17.5 min处有一未知物质,其UV信号比较强,且与谷氨酸积累相关,通过高压液相和串联质谱,该物质鉴定为N-乙酰-谷氨酰胺.对比分析不同发酵过程,N-乙酰-谷氨酰胺积累与谷氨酸偶联,且与谷氨酸产量呈相关.溶氧对N-乙酰-谷氨酰胺合成没有显著影响,玉米浆是影响其合成的...
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| 关 键 词： | 谷氨酸 N-乙酰-谷氨酰胺 发酵 玉米浆 |
N-acetyl-L-glutamine Accumulation during Glutamic Acid Production |
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| ZHAO Pu-ya,HUANG Lei,SUN Cun-min,ZEGN Xiang-feng,JIA Shi-ru,SUN Ji-bin.N-acetyl-L-glutamine Accumulation during Glutamic Acid Production[J].Modern Food Science & Technology,2011(9):1057-1061. |
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| Authors: | ZHAO Pu-ya HUANG Lei SUN Cun-min ZEGN Xiang-feng JIA Shi-ru SUN Ji-bin |
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| Affiliation: | ZHAO Pu-ya1,2,HUANG Lei3,SUN Cun-min2,ZEGN Xiang-feng2,JIA Shi-ru1,SUN Ji-bin2(1.College of Bioengineering,Tianjin University of Science & Technology,Tianjin 300457,China)(2.Key Laboratory of Systems Microbial Biotechnology,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China)(3.Shandong Public Security Bureau,Jinan 250001,China) |
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| Abstract: | By HPLC analysis of samples from glutamic acid fermentations,an unknown chemical was found to accumulate in glutamic acid production at the retention time of 17.5 minute.This chemical was identified as N-acetyl glutamine based on the HPLC-tandem mass spectrometry analysis and approved by using commercial compounds.N-acetyl-glutamine formation was coupled with the glutamic acid production positively and almost linearly with the prolongation of the fermentation processes.Comparative analysis of different ferm... |
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| Keywords: | glutamic acid acetyl glutamine fermentation corn steep liquor |
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