膳食纤维强化冻干山楂果对小鼠肠道健康的影响

本文研究了添加10%山楂膳食纤维的冻干山楂果(按质量比9:1)对小鼠肠道健康的影响。将小鼠按体重质量随机分为4组,分别为溶剂对照组和受试物1、2和4 g/(kg·d)剂量组。灌胃6周后,试验结束前连续3 d收集小鼠新鲜粪便,测定粪便质量、水分、pH值和短链脂肪酸(SCFA)含量。试验结束空腹过夜条件下测体重,处死小鼠,分离盲肠和结肠并测定质量;取盲肠和结肠内容物测定pH和盲肠酵解产物SCFA的含量;测定盲肠和结肠内容物中的双歧杆菌、肠杆菌、肠球菌、乳杆菌和产气荚膜梭菌的数量。分析添加10%山楂膳食纤维的冻干山楂果对小鼠肠道健康的影响。各剂量组与溶剂对照组相比,各项检测指标存在显著性差异(p0.05),结果表明添加10%山楂膳食纤维的冻干山楂果对小鼠肠道内环境健康具有改善调节作用。

Effect of Increased Dietary Fiber in Freeze-dried Hawthorn on the Intestinal Health of Mice

The ameliorative effect of freeze-dried hawthorn fruit with added dietary fiber (mass ratio 9:1) on mouse intestinal health was investigated. The mice were divided into 4 groups: one blank control group and three dose groups given 1, 2, and 4 g/(kg?d) of hawthorn fruit with 10% dietary fiber. After intragastric gavage for 6 weeks, fresh feces were collected for three consecutive days before the end of the trial, and the weight, moisture content, pH, and short-chain fatty acid (SCFA) content of the feces were measured. At the end of the trial, the body weight of the mice was measured after overnight fasting. The mice were then sacrificed, and the caecum was separated from the colon and weighed. The cecum and colon contents were extracted, and their pH and SCFA content were measured. The counts of Bifidobacterium, Enterobacter, Enterococcus, Lactobacillus, and Clostridium perfringens in the cecum and colon contents were determined. Compared with the control group, the test indices for all three dose groups showed significant differences (p<0.05). The results showed that adding 10% hawthorn dietary fiber to the freeze-dried hawthorn fruit could improve the intestinal environment and health of mice.