质子诱导AHH-1细胞PIG3基因的mRNA表达和细胞周期改变研究

本文利用串列加速器加速的21MeV的质子束流对人正常淋巴细胞AHH-1进行辐照,研究了质子诱导AHH-1细胞PIG3基因的mRNA表达量和细胞周期改变的剂量和时间相关性。实时定量聚合酶链式反应(PCR)结果显示,质子辐照后,PIG3基因mRNA的表达量随剂量的增大而增大,表现出很好的剂量相关性。在不同的剂量点下,表达量随时间的变化趋势也大致相似,峰值出现的时间点除8Gy剂量点出现在12h外,其他剂量点均在照后6h达到。理论拟合曲线和实验数据也呈现出很好的一致性。细胞周期检测结果显示,在每个剂量点下,G2/M期细胞随着时间的延长呈现先增加后减少的趋势。以上结果说明PIG3基因mRNA的表达量在生物剂量计的应用上可能具有一定的基础和潜力,值得进一步研究。

mRNA Expression of Genes PIG3 and Cell Cycle Change of AHH-1 Cell Induced by Proton

AHH-1 cell was irradiated with proton (21 MeV) at Q3D magnetic spectro-meter of the tandem accelerator to study the dose and time-effect of PIG3 gene mRNA expression in AHH-1 cell. Real-time quantitative PCR results show that PIG3 gene mRNA expression increases with doses at the different time after proton irradiation. And the PIG3 gene mRNA expression changes along with time at each dose. The peak of the PIG3 gene mRNA expression appears at 6 h for every dose except the 8 Gy at 12 h. The results of cell cycle show that the percent of G2/M first increases and then decreases with time. These results show that PIG3 gene mRNA expression in the application of biological dosimeter is potential.