| 脑M受体生成和降解速率测定方法的建立 |
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| 引用本文： | 胡雅儿,潘镇球.脑M受体生成和降解速率测定方法的建立[J].核技术,1991,14(5):285-290. |
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| 作者姓名： | 胡雅儿 潘镇球 |
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| 作者单位： | 上海第二医科大学基础医学院，上海第二医科大学基础医学院，上海第二医科大学基础医学院，浙江医科大学药学系，浙江医科大学药学系，上海第二医科大学基础医学院 90届毕业实习生，90届毕业实习生 |
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| 摘 要： |
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| 关 键 词： | 脑 M受体 更新 生成速率 |
The determination of the production and degradation rate of brain M-cbolinergic receptors |
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| Hu Yaer Xia zongqin Bian Yijie Pan ZhenqiuPan Xiaohong Yi Ningyu.The determination of the production and degradation rate of brain M-cbolinergic receptors[J].Nuclear Techniques,1991,14(5):285-290. |
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| Authors: | Hu Yaer Xia zongqin Bian Yijie Pan ZhenqiuPan Xiaohong Yi Ningyu |
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| Abstract: | A technique for the determination of the production and degradation rates of mouse brain M-receptors was established. The selective alkylating ager.t BCM was given in vivo to irreversibly inhibit the M-receptors and the 3H-QNB binding assay was used to monitor the time-related increase of newly synthesized receptors. A tracer kinetics mathematical model was used to calculate the parameters. The results of three separate experiments with Balb-C mice were close to each other, the average production rate being 37.34fmol/mg protein/h, the average degradation rate constant being 0.035/h, and the half-time being 19.82h. The significance of this technique in the study of the mechanism of pathological changes in brain M-receptors and drug actions was discussed. |
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| Keywords: | M receptor turnover M receptor synthesis rate Benzilylcholine mustard Irreversible antagonists |
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