不良心理应激对小鼠人卵巢癌移植瘤dicer酶表达的影响

目的观察不良应激因素对小鼠人卵巢癌移植瘤dicer酶的表达影响。方法将人卵巢癌组织采用背部皮下接种法移植裸鼠,建立人卵巢癌细胞裸鼠皮下移植瘤模型。成功造模后,取体质量及肿瘤大小相近的裸鼠按随机数字表法分为对照组和应激组,每组6只。对照组裸鼠自由供水和食物;应激组裸鼠采用束缚方法给予应激,束缚应激时将应激组裸鼠依次放入打孔通风的50 mL离心管,每天束缚6 h,5 d.周-1,束缚期间禁水、禁食,完成束缚应激后将裸鼠放出,恢复自由供水和食物,应激时间共2周。观察2组裸鼠生存状态、体质量及皮下移植瘤体积;完成应激后处死裸鼠,取皮下人卵巢癌组织,采用RT-PCR和免疫组织化学染色,比较2组人卵巢癌组织中dicer酶的表达水平。结果应激组裸鼠摄食及活动不佳,与对照组相比,应激组裸鼠体质量明显下降（P〈0.05）;2组比较,肿瘤体积差异无统计学意义（P〉0.05）;RT-PCR和免疫组织化学染色结果表明：与对照组相比,应激组裸鼠卵巢癌组织中dicer酶的表达量明显下降（P〈0.05）。结论不良心理应激因素能降低人卵巢癌移植瘤中dicer酶的表达。dicer酶的表达可成为研究不良心理应激对肿瘤生长影响的重要参考指标。

Effect of Dys-psychological Stress on Expression of Dicer in Mice with Human Ovarian Cancer Xenografts

Objective To observe the effect of dys-psychological stress on the expression of dicer in mice with human ovarian cancer xenografts.Methods The subcutaneous tumor xenografts were established by implanting human ovarian cancer tissue into nude mice.After establishment of the model,the mice with similar weight and diameter of tumors were randomly divided into control group and stress group,with 6 mice in each group.The dys-psychological stress model was established by putting each nude mouse respectively into a 50 mL centrifuge tube with drilling for excellent ventilation.Six hours later,the mice were released from the tubes and then supplied with food and water.The mice with dys-psychological stress were restrained 6 hours per day for two weeks.Control mice were given free access to water and food.The survival status,body mass and tumor size were observed in both groups.After the restraint course,the ovarian cancer tissues were collected from the mice and the expression of dicer was detected by RT-PCR and immunohistochemistry.Results The mice in stress group had poor feeding and activity.Compared with control mice,the body mass and dicer expression were significantly decreased by dys-psychological stress（all P0.05）.There were no differences in tumor volume between control group and stess group（P0.05）.Conclusion Dys-psychological stress was negatively correlated with the expression of dicer in human ovarian cancer xenografts,and the level of dicer expression is an important index for investigating the effect of dys-psychological stress on the development of ovarian cancer.