青黛散外用凝胶对特异性皮炎小鼠的改善作用研究

目的：研究青黛散凝胶（Qingdaipowder Gel, QDPG）对小鼠特异性皮炎（AD）模型的影响及青黛散醇提物的抑菌作用。方法：采用2，4-二硝基氯苯（DNCB）反复皮肤诱导建立小鼠AD模型，将56只小鼠随机分为空白组、模型组、尤卓尔组（Hyd，1.5 mg/cm2）、基质组、青黛散凝胶高剂量组（QDPG-H，240 mg/cm2）、青黛散凝胶中剂量组（QDPG-M，120 mg/cm2）、青黛散凝胶低剂量组（QDPG-L，60 mg/cm2）剂量组。外用皮肤给药，每日1次，连续12 d，分析各组小鼠皮损、耳廓肿胀度、胸腺脾脏指数，苏木素-伊红（HE）染色观察皮损组织的病理变化，酶联免疫吸附测定法（ELISA）检测血清中免疫球蛋白E（IgE）、白细胞介素6（IL-6）、白细胞介素4（IL-4）、肿瘤坏死因子α（TNF-α）和γ干扰素（IFN-γ）的炎症因子水平。采用滤纸片法和最小抑菌浓度（MIC）法，确定青黛散醇提物对特异性皮炎主要致病菌金黄色葡萄球菌、大肠埃希氏菌的抑菌活性。结果：与模型组相比，Hyd组和QDPG各剂量组耳廓肿胀度显著降低（P<0.01）、皮层厚度显著降低（P<0.01），血清中IL-4、IL-6、IgE、IFN-γ和TNF-α水平显著降低（P<0.01），IFN-γ/IL-4的比值升高（P<0.05），QDPG-H剂量组胸腺脾脏指数降低（P<0.05）。青黛散醇提物对大肠埃希氏菌和金黄色葡萄球菌的MIC值分别为31.25 mg/mL和62.5 mg/mL。 结论：QDPG能有效缓解特异性皮炎的症状，其作用机制可通过抑制致病菌、调节T辅助细胞TH1/TH2的平衡，改善AD模型小鼠的皮损。

Study on the improvement of Qingdaipowder Gel for external use on mice with specific dermatitis

AIM: To study the effect of Qingdaipowder Gel (QDPG) on mice specific dermatitis (AD) model and the antibacterial effect of the ethanol extract of Qingdaipowder. METHODS: AD model of mice was established by repeated skin induction with 2,4-dinitrochlorobenzene (DNCB). Fifty-six mice were randomly divided into blank group, model group, Hydrocortisone Butyrate Cream group (Hyd, 1.5 mg/cm2), matrix group, high dose group of Qingdaipowder Gel (QDPG-H, 240 mg/cm2), medium dose group of Qingdaipowder Gel (QDPG-M, 120 mg/cm2), and low dose group of Qingdaipowder Gel (QDPG-L, 60 mg/cm2). For external use, the drug was administered to the skin once a day for 12 consecutive days. The skin lesions, auricle swelling, thymus and spleen index of mice in each group were analyzed. Hematoxylin eosin (HE) staining was used to observe the pathological changes of skin lesions, and enzyme-linked immunosorbent assay (ELISA) was used to detect serum immunoglobulin E (IgE), interleukin-6 (IL-6), interleukin-4 (IL-4), and tumor necrosis factor α (TNF-α) and γ Interferon (IFN-γ) Level of inflammatory factors. Filter paper method and minimum inhibitory concentration (MIC) method were used to determine the antibacterial activity of the ethanol extract of Qingdaipowder against Staphylococcus aureus and Escherichia coli, the main pathogens of specific dermatitis. RESULTS: Compared with the model group, the degree of auricle swelling and the thickness of cortex in Hyd group and QDPG groups decreased significantly (P<0.01), and the serum levels of IL-4, IL-6, IgE, IFN-γ And TNF-α The level decreased significantly (P<0.01), IFN- γ/The ratio of IL-4 increased (P<0.05), and the thymus spleen index decreased (P<0.05) in QDPG-H group. The MIC values of the ethanol extract of Qingdaipowder against Escherichia coli and Staphylococcus aureus were 31.25 mg/mL and 62.5 mg/mL, respectively. CONCLUSION: QDPG can effectively alleviate the symptoms of specific dermatitis, and its mechanism of action can improve the skin lesions of AD model mice by inhibiting pathogenic bacteria and regulating the balance of T helper cells TH1/TH2.