开口箭不同提取部位对异丙肾上腺素致小鼠心肌缺血损伤的影响

目的: 研究开口箭不同提取部位对异丙肾上腺素(ISO)致小鼠心肌缺血损伤的保护作用。方法: 将105只小鼠随机分为正常组、模型组、开口箭石油醚、乙酸乙酯、正丁醇、水部位组和地奥心血康组;各给药组分别灌胃给予相应的药物,正常组及模型组给予等容量的 0.5%CMC-Na灌胃,给药 7 d 后,随后 3 d 在给药后 30 min,模型组、开口箭提取物组和地奥心血康组皮下注射ISO 5.0 mg/kg,正常组则皮下注射同体积的生理盐水。给药结束次日取血处死小鼠,测定心脏脏器指数;左心室匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧物酶(GSH-Px)、丙二醛(MDA)水平;血浆中谷草转氨酶(AST)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、肌酸激酶同功酶(CKMB)、羟丁酸脱氢酶(HBDH)活力,另取心脏组织,分别进行心脏组织形态学和超微结构分析。结果: 开口箭不同提取部位均可显著逆转ISO升高的心脏脏器指数,心肌中异常的MDA含量及SOD、GSH-Px水平,明显降低血液中升高的AST、LDH、HBDH、CK、CKMB水平,降低心肌炎性浸润、溶解坏死和胶原纤维面积,与模型组比较,开口箭正丁醇和乙酸乙酯部位均具有统计学差异(P＜0.05,P＜0.01),其治疗效果与地奥心血康相当;石油醚部位在改善HBDH、SOD等方面有统计学差异(P＜0.05);水部位除LDH、CK、GSH-Px外,均具有统计学差异(P＜0.05)。结论: 开口箭不同提取部位对ISO致小鼠心肌缺血损伤具有较强的保护作用,其作用效果由强至弱依次为:开口箭正丁醇部位、乙酸乙酯部位、水部位和石油醚部位。

Effects of different fractions extracted from Tupistra chinensis Bak on isoproterenol- induced myocardial injury in mice

AIM: To investigate the effects of different fractions extracted from Tupistra chinensis Bak on isoproterenol (ISO)-induced myocardial injury in mice.METHODS: 105 KM mice were evenly and randomly divided into normal group, model group, four different fractions extracted from Tupistra chinensis Bak groups and Di'aoxinxuekang group. The treatment groups were orally treated homologous medicines, the normal and model groups were given the same volume of 0.5% CMC-Na. After the treatment of 30 min on the subsequently 3 days, the model group, four different fractions extracted from Tupistra chinensis Bak groups and Di'aoxinxuekang group were injected subcutaneously with ISO (5 mg/kg), while the animals in normal group were received injection of normal saline. Blood samples from mice on the next day after the last administration were collected plasm levels of AST, LDH, HBDH, CK and CKMB. The mice were then sacrificed under anesthesia to collect myocardial tissues for measurements of heart weight index (heart weight/body and left ventricles weight/heart weight), myocardial SOD,GSH-Px activities and MDA content, and analysis of heart tissue morphology and ultra microstructure.RESULTS: The different fractions extracted from Tupistra chinensis Bak might significantly reversed the ISO-induced heart index, abnormal higher myocardial tissue MDA contents and less SOD, GSH-Px activities, and also reduced abnormal higher plasm AST, LDH, HBDH, CK and CKMB levels. The myocardial inflammatory infiltration, lytic necrosis, collagen fiber area were remarkably alleviated due to the four fractions. The effects of the n-butyl alcohol and ethyl acetate fractions were significant as compared with the model group (P＜0.05, P＜0.01), and the effect of treatment with Di'aoxinxuekang quite; Petroleum ether fraction revealed a statistically significant in improving HBDH and SOD levels compared with the model group (P＜0.05), and that of water fraction was also significant as compared with the model group except for in the case of LDH, CK and GSH-Px (P＜0.05).CONCLUSION: The treatments with different fractions extracted from Tupistra chinensis Bak have remarkably protective effect. The effects from strong to weak are as follows: the n-butyl alcohol fraction, ethyl acetate fraction, water fraction and petroleum ether fraction.