抗衰灵膏延缓衰老作用及其机制研究

目的: 观察抗衰灵膏延缓衰老作用，并初步探讨其作用机制。方法: ICR小鼠，随机分为正常组、模型组、阳性组（延寿片）、抗衰灵膏高、中、低组。背部皮下注射5%D-半乳糖，建立小鼠亚急性衰老模型，各组动物灌胃给相应药物6周后，测定脾脏和胸腺指数；酶联免疫法（ELISA）测定脑组织中过氧化脂质（LPO）、脂褐素（LF）、B型单胺氧化酶（MAO-B）含量和活性；紫外分光光度法测定血清中丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）含量及活性；HE染色观察脑组织病理改变。结果: 与模型组比较，抗衰灵高、中、低剂量明显增加脾脏系数，高、中剂量可明显增加胸腺系数；高剂量可明显降低脑组织中LPO、LF含量，高、中、低剂量组可明显抑制脑组织中MAO-B活性的降低；高、中、低剂量可明显降低血清中MDA含量，高、中剂量可明显升高血清中SOD、GSH-Px活力；高、中剂量可明显改善脑组织病理改变。结论: 抗衰灵膏具有较好的延缓衰老作用，其机制可能与抗氧化有关。

Anti-aging effects of Kangshuailing ointment and its mechanism

AIM: To observe the anti-aging effects of Kangshuailing ointment and the possible mechanisms. METHODS: ICR mice were randomly divided into six groups: normal group, model group, positive group, Kangshuailing ointment 1.3, 2.6, 5.2 g/kg group. Aging model was induced by subcutaneously injecting mice with 5% D-galactose. Mice of each group receiced daily administration by gavage for 6 weeks. Thymus index and spleen index were calculated; LPO, LF contents and MAO-B activity in brain tissue were detected by ELISA method; MDA contents, SOD and GSH-Px activities in blood serum were examined by spectrophotometry method; the pathology change of brain tissues were observed using light microscope. RESULTS: Compared with model group, Kangshuailing ointment could significantly increase the thymus and spleen index, reduce the LPO and LF contents, and increase MAO-B activity in brain tissue, remarkably decrease the MDA contents and increase the SOD and GSH-Px activities in blood serum. In addition, Kangshuailing ointment could markedly relieve the pathological lesions induced with D-galactose in brain. CONCLUSION: Kangshuailing ointment has obvious anti-aging effect on aging mice, which may be related to the antioxidation.