汉防己甲素对硫唑嘌呤致大鼠肝损伤的保护作用

目的 研究汉防己甲素(Tet)对硫唑嘌呤(Aza)致大鼠肝损伤的保护作用。方法 大鼠分为三组:①对照组;②Aza 组;③Aza+Tet 组。给药量分别为Aza 25mg/kg·d。Tet 30mg/k g·d,测定用药1wk 和2wk 时大鼠血清谷丙转氨酶(ALT)、碱性磷酸酶(AKP)、总蛋白(TP)和白蛋白(Alb)等肝功能指标以及血清中丙二醛(MDA)、超氧化物歧化酶(SOD)和全血谷胱甘肽含量(GSH), 并作病理观察。结果 用药1wk 及2wk 后, Aza 组大鼠血清A LT、AKP、MDA 含量均显著升高, TP、Alb、SOD、GSH 含量均显著降低;合用Tet 组则上述变化不明显, 与对照组比较无显著性差异。病理学检查发现合用Tet 组病理改变较轻微。结论 Tet 对Aza 致大鼠肝损伤有保护作用, 推测此作用与其抗脂质过氧化物、增加内源性解毒物质有关。

Protective effects of tetrandrine on azathioprine hepatotoxicity in rats

Aim To study the protective effects of tetrandrine (Tet)on azathioprine (Aza)hepato toxicity in rats.Method 27 rats were separated into 3 groups, and Aza 25 mg/kg·d, Aza 25 mg/kg·d+Tet 30mg/kg·d and normal saline were separately given to the rats in each of the 3 groups.The parameters of liver function, malondialdehyde (MDA)and surperox ide dismutase (SOD)in plasma, and glutatione (GSH)in the whole blood were detected in all.The patho logical changes of rats liver were observed by light microscope.Result In the Aza group, the levels of ALT, AKP and MDA were increased and those of total protein, albumine, SOD and GSH were decreased significantly at 1 wk and 2 wk.In the Tet group, the levels of ALT, MDA and AKP were increased and those of SOD and GSH were not decteased significantly.The hepatocy tes degene ration and necrosis in Aza group were found under light microscope.The damages in Tet group were very little.Conclusion Tet had protective effects on Aza hepatotoxicity in rats.