骨组织工程用硅胶/掺锶β-磷酸三钙/硫酸钙复合多孔支架的制备与性能研究

以掺锶β-磷酸三钙/硫酸钙为原料,利用搅拌喷雾干燥法制备出掺锶β-磷酸三钙/硫酸钙复合小球,再将硅胶与制备的复合小球复合,通过在模具中堆垛聚集的方法,制备出硅胶/掺锶β-磷酸三钙/硫酸钙复合生物支架。采用XRD,SEM,FT-IR等方法分析制得复合多孔支架的成分、形貌以及结构特征,并研究复合生物支架的降解性、孔隙率、力学性能和细胞毒性等。结果表明:该复合多孔生物支架具有一定的不规则孔洞结构,小球与小球之间的孔隙约为0.2~1mm,而每个小球上也有大量的微孔,孔径在50~200μm之间,且平均孔隙率达到62%,基本能满足骨组织工程支架对孔隙率的要求;该复合多孔支架无细胞毒性,其降解周期约为80天,抗压强度约为0.1MPa,因此该支架在非承重骨组织修复方面具有良好的应用前景。

Fabrication and Properties of Silica Gel/Calcium Sulfate/Strontium-doped β-tricalcium Phosphate Composite Porous Scaffolds for Bone Tissue Engineering

The calcium sulfate/strontium-doped β-tricalcium phosphate composite spherical pellets was fabricated,using the calcium sulfate/strontium-doped β-TCP as raw material,and through the stirring spray drying method,and then composite spherical pellets were combined with silica gel,porous silica gel/calcium sulfate/ strontium-doped β-tricalcium phosphate scaffold was obtained by stacking aggregation method in the mould.The XRD,SEM and FT-IR,etc are employed to examine the chemical composition,composite morphology and structure characteristics,and the degradability,porosity,mechanical properties and cytotoxicity of the scaffolds materials were studied.The results reveal that the composite porous scaffolds have irregular pore structure with pore size between 0.2-1.0mm,and they have a large number of micropores on each of the composite spherical pellets,with the aperture between 50-200μm.Moreover,the porosity of the composite scaffolds is about 62％,which can meet the requirements of scaffolds for bone tissue engineering in porosity;the cytotoxicity tests show the composite scaffolds have no cytotoxic effect and it has good degradation.Therefore,it has good application prospect in bone tissue engineering of the bone defect repair of non-bearing site.