Analysis of marine hydrobiont lipid extracts as possible cryoprotective agents |
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| Authors: | NA Odintsova NV Ageenko KV Kiselev NM Sanina EY Kostetsky |
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| Affiliation: | aInstitute of Marine Biology, Far East Branch of RAS, 690041 Vladivostok, Russian Federation;bInstitute of Biology and Soil Science, Far East Branch of RAS, 690022 Vladivostok, Russian Federation;cFar Eastern National University, 690090 Vladivostok, Russian Federation |
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| Abstract: | We studied a combined effect of the cryoprotectants both of lipid and carbohydrate origin at the presence of DMSO or without it on cell viability and the RNA synthesis in the embryonic mollusc and echinoderm cell cultures obtained at different developmental stages. Cryoprotective properties of exogenous lipids correlated with their thermotropic behavior. Lipid extracts from marine hydrobiontes, which thermal transitions are characterized by relatively small part of calorimetric thermogram area lying at temperatures above 0 °C (6–18%), were found to possess a considerable cryoprotective activity. The results are discussed in relation to the prediction of the cryoprotective activity of lipid extracts for marine animal cells. In addition, it was found that marine invertebrate cells can be cryopreserved without DMSO at the presence of trehalose and lipid extracts of some marine hydrobiontes. |
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| Keywords: | Cryogenics Cryoprotectant Crypreservation Cell Mollusc Sturgeon Lipid Embryo DMSO Transition PhaseMots clé s: Cryogé nie Cryoprotecteur Cryoconservation Cellule Mollusque Esturgeon Lipide Embryon DMSO Transition Phase |
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