骨修复用聚磷酸钙/壳聚糖复合材料的合成及其细胞相容性

采用自行设计的悬浮液热分散复合法, 用硬脂酸(Sad)作为致孔剂, 通过复合-沉析-浸溶-漂洗-干燥的工艺制备大孔聚磷酸钙/壳聚糖(CPP/CS)复合材料棒材。将复合悬液滴入凝固液中, 经浸泡-漂洗-干燥的工艺制备CPP/CS微孔复合材料颗粒。用红外光谱及扫描电镜对复合材料进行了表征。实验表明, 合成的复合材料中CS的氨基和CPP的P O基生成了氢键, 其形态结构致密均匀, 大孔复合材料棒材的孔径为50~300μm, 孔隙率为71.13%; 微孔复合材料颗粒的孔径为10~100μm, 孔隙率为40.76%。研究了2种工艺不同配比复合材料的细胞相容性, 发现: 大孔复合材料棒材的细胞相容性比微孔复合材料颗粒好, 复合材料中随着CPP含量的增加, 细胞相容性增加, 当复合材料中CPP和CS的质量比为7/3时, 复合材料的细胞相容性较好。CPP与CS复合可以提高其压缩强度, 原料质量配比为CPP/CS＝7/3时, 复合材料的强度最高。

Syntheses and cell compatibilities of CPP/CS composite for bone repair

A series of macroporous calcium polyphosphate/chitosan(CPP/CS) composite rod and microporous CPP/CS composite particle were synthesized with CPP and CS using two technologies by a heat-dispersion suspension complex method. The structure and morphology of the composites were measured by IR spectrum and SEM. The hydrogen bond was formed between —NH2 of CS and —P O of CPP in the composites. The aperture of the macroporous composite is about 50~300μm, the porocity is 71.13%, which was determined by the ethanol infiltration method. And for the microporous composites, the aperture and porocity are 10~100μm and 40.76%, respectively. The former is suitable for the need of bone tissue engineering scaffold material, but the later is not suitable. The cell compatibility of macroporous composite was better than that of microporous composite. The cell compatibility is the best when CPP/CS=7/3. The compression strength of CPP is increased by the complex method. And the compression strength of composite is also the highest when CPP/CS=7/3.