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| 拟除虫菊酯类农药单克隆抗体的制备及鉴定 | |
| 引用本文： | 徐敦明,卢声宇,周昱,杨方,林立毅,杜凤君,张缙.拟除虫菊酯类农药单克隆抗体的制备及鉴定[J].福建分析测试,2010,19(4):5-9. |
| 作者姓名： | 徐敦明 卢声宇 周昱 杨方 林立毅 杜凤君 张缙 |
| 作者单位： | 1. 厦门出入境检验检疫局,福建,厦门,361026;福建出入境检验检疫局,福建,福州,350001 2. 福建出入境检验检疫局,福建,福州,350001 3. 厦门出入境检验检疫局,福建,厦门,361026 |
| 基金项目： | 福建省青年人才项目(，国家质检总局科技项目，厦门市科技计划项目 |
| 摘 要： | 以间苯氧基苯甲酸(PBA)为半抗原,与牛血清白蛋白(BSA)偶联后作为抗原,免疫Balb/c小鼠,取小鼠脾细胞与骨髓瘤细胞(SP2/0)融合,使用PBA与鸡卵清白蛋白(OVA)偶联物作为包被物,间接ELISA法筛选到4株特异性针对PBA的杂交瘤。其中两株细胞2F7和7B1制备腹水并用ProteinG柱纯化抗体,抗体效价均达到1:625000。单抗7B1用于间接竞争ELISA法,PBA检测下限为0.025μg·mL-1,IC50为0.57μg·mL-1,对溴氰菊酯、甲体氯氰菊酯、甲氰菊酯、氰戊菊酯均有特异性识别,IC50分别为0.79μg·mL-1、0.74μg·mL-1、0.63μg·mL-1、0.8μg·mL-1,对联苯菊酯识别弱,IC50为99.7μg·mL-1。该单抗可用于多种菊酯类农药的检测。 |
| 关 键 词： | 拟除虫菊酯 农药残留 单克隆抗体 酶联免疫检测方法 |
Preparation and Characterization of Monoclonal Antibodies against Pyrethroid Pesticides |
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| Xu Dun-ming,Lu Sheng-yu,Zhou Yu,Yang Fang,Lin Li-yi,Du Feng-jun,Zhang Jin.Preparation and Characterization of Monoclonal Antibodies against Pyrethroid Pesticides[J].Fujian Anal Ysis & Testing,2010,19(4):5-9. | |
| Authors: | Xu Dun-ming Lu Sheng-yu Zhou Yu Yang Fang Lin Li-yi Du Feng-jun Zhang Jin |
| Affiliation: | Xu Dun-ming1,2,Lu Sheng-yu2,Zhou Yu1,Yang Fang2,Lin Li-yi1,Du Feng-jun1,Zhang Jin1 (1. Xiamen Entry-Exit Inspection and Quarantine Bureau,Xiamen,Fujian361026,China,2.Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou,Fujian350001,China) |
| Abstract: | Enzyme immunoassay was a desirable method for detecting residues of pyrethroids insecticides. Recently many immunoassays methods for pyrethroids have been reported, but few focused on the multi-residue analysis of pyrethroids. To develop a monoclonal antibodies-based determination method for multi-residues of pyrethroids, a generic hapten, 3-phenoxybenzoic acid (PBA)was used to generate broad specificity monoclonal antibodies against a group of pyrethroids. Mixed anhydride reaction (MAR)and active ester met... |
| Keywords: | pyrethroid pesticide residue monoclonal antibody enzyme immunoassay |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
