梯度洗脱HPLC-ELSD法测定蛋黄卵磷脂有关物质

目的：提升蛋黄卵磷脂有关物质的检测方法。方法：采用高效液相法,色谱柱：Grace Alltima Silica（4.6mm＊250mm＊5μm）色谱柱;流动相：以甲醇-水-冰醋酸-三乙胺（85∶15∶0.45∶0.05）为流动相A,以正己烷-异丙醇-流动相A（20：48：32）为流动相B,梯度洗脱;检测器为蒸发光检测器（气体流速：2.5ml/min,增益：16,漂移管温度：50℃）;流速：1.0 mL·min^-1,柱温：40℃。结果：蛋黄卵磷脂各杂质之间均达到良好分离;溶液稳定性良好;各杂质加样回收率良好。结论：该法快速、简便、准确、重复性好,适用于蛋黄卵磷脂的质量控制。

Determination of related substances in Egg Yolk Lecithin by HPLC-ESLD with gradient elution

Objective：Improve the detection method of related substances in egg yolk lecithin.Method：The HPLC was carried out on a Grace Alltima Silica（250 mm×4.6 mm,5μm）column with a flow rate of 1.0 ·min^-1 at column temperature 40℃with evaporative light scattering detector;The mobile phase consisted of Methanol：water：acetic acid：three triethylamine（85∶15∶0.45∶0.05）as the mobile phase A and hexane ：isopropanol ：mobile phase A（20：48：32）as the mobile phase B,Gradient elution was used;The gas flow was 2.5ml/min,gain was 16,the temperature of evaporative pipe was 50℃;Results：The of egg yolk lecithin were well separated;The stability of solution was good;The average recoveries of all impurities was good.Conclusion：It is an accurate,simple,rapid,qualitative method for the determination of related substances in egg yolk lecithin.