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| 天麻抗真菌蛋白(GAFP)的N端序列测定及cDNA基因克隆 | |
| 引用本文： | 王义琴,李文彬,Lam Honming,张秀海,陈潜,郭顺星,孙勇如.天麻抗真菌蛋白(GAFP)的N端序列测定及cDNA基因克隆[J].高技术通讯,2000,10(1):10-14. |
| 作者姓名： | 王义琴 李文彬 Lam Honming 张秀海 陈潜 郭顺星 孙勇如 |
| 作者单位： | 1. 中科院遗传研究所,北京,100101 2. 香港中文大学,香港,沙田新界 3. 中国医学科学院药用植物研究所,北京,100094 |
| 基金项目： | 国家自然科学基金!资助项目 ( 3 9870 40 5 ) |
| 摘 要： | 从新鲜天麻次生块茎中将天麻抗真菌蛋白（ＧＡＦＰ）提纯，然后测得其Ｎ端的３０个氨基酸，根据氨基酸序列设计了一段３０ｂｐ的核酸探针，从ｃＤＮＡ文库中筛选到了编码该蛋白的基因，为这一新型蛋白在抗真菌基因工程中的应用奠定了基础。 |
| 关 键 词： | 天麻 cDNA文库 N端序列 抗真菌蛋白基因 |
N-terminal Sequence and cDNA Cloning of Gastrodia Antifungal Protein (GAFP) from Gastrodia ( G. elata BI f . elata) |
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| Wang Yiqin,Li Wenbin,Lam Honming,Zhang Xiuhai,Chen Qian,Guo Shunxing,Sun Yongru.N-terminal Sequence and cDNA Cloning of Gastrodia Antifungal Protein (GAFP) from Gastrodia ( G. elata BI f . elata)[J].High Technology Letters,2000,10(1):10-14. | |
| Authors: | Wang Yiqin Li Wenbin Lam Honming Zhang Xiuhai Chen Qian Guo Shunxing Sun Yongru |
| Abstract: | Gastrodia antigungal protein(GAFP) extracted from the traditional Chinese medicine Gastrodia (G.elata BI f. elata) , which displays strong antifungal activity to several fungi,was purified, and the N terminal was sequenced. According to the sequence of ammonia acid, a probe of 30bp was designed. Using this probe to screen the cDNA library constructed from the secondarycorm, the encoding GAFP gene was got. The study laid a foundation for further application of this antifungal protein to the genetic engineering. |
| Keywords: | Gastrodia secondarycorm N terminal sequence cDNA library GAFP gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
