| 恶性疟原虫FCC1／HN株P190抗原保守区基因的克隆及其在大肠杆菌中的表达 |
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| 引用本文： | 杨树桐,潘卫庆.恶性疟原虫FCC1／HN株P190抗原保守区基因的克隆及其在大肠杆菌中的表达[J].高技术通讯,1994,4(3):32-35. |
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| 作者姓名： | 杨树桐 潘卫庆 |
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| 作者单位： | 上海第二军医大学医学生物技术和分于遗传研究所 |
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| 基金项目： | 联合国开发计划署,世界银行,世界卫生组织 |
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| 摘 要： | Ｐ１９０是恶性疟原虫裂殖子表面抗原，是很有希望的疟疾疫苗候选抗原。采用ＰＣＲ方法克隆了我国海南省ＦＣＣ１／ＨＮ株Ｐ１９０抗原基因的３个保守区片段，连接到ｐＵＣ１８载体上进行ＤＮＡ序列测定，然后分别与表达载体ｐＧＥＸ－２Ｔ连接，经双酶切鉴定后转化感受态ＪＭ１０９大肠杆菌进行高效融合表达。
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| 关 键 词： | 恶性疟原虫 P190抗原 基因表达 |
Cloning and Expression of Three Conserved Regions on P190Antigen of Plasmodium Falciparum in E. Coli |
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| Yang Shutong, Pan Weiqing, Deng Hailin, Lu Deru.Cloning and Expression of Three Conserved Regions on P190Antigen of Plasmodium Falciparum in E. Coli[J].High Technology Letters,1994,4(3):32-35. |
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| Authors: | Yang Shutong Pan Weiqing Deng Hailin Lu Deru |
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| Affiliation: | (received Nov. 3,1993 )Yang Shutong, Pan Weiqing, Deng Hailin, Lu Deru(Institute of Medical Biotechnology and Molecular Genetics,Shanghai,2004 33 ) |
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| Abstract: | 90-kilodalton glycoprotein (P190) of Plasmodium falciparum, precursor of the major surface proteinof merozoites, is considered a promising candidate for blood stage malaria vaccine. We designed six primersaccording to the sequence of MAD20 strain. Three DNA fragments, named as P190 CR2-2, P190 CR3,P190 CR4, respectively, which encoded amino acid residues of conserved region , and on theP190 Antigen, were amplified by polymerase chain reaction from genomic DNA in FCC1/HN strain ofplasmodium falciparum isolated from Hainan Province, China. The amplified fragments were subclonedinto pUC18 vector and sequenced. Those three sequenced fragments inserted into pGEX-2T plasmid . E.colt JM109 (DE3 ) were transformed with the recombinant plasmids. The results show that the threefragments were expressed at high level as C-terminal fusions with glutathione S-trabsferase. |
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| Keywords: | : Plasmodium falciparum Merozoite surface antigen 1 Malaria vaccine |
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