Characterization of Non-crosshybridizing DNA Oligonucleotides Manufactured in vitro |
| |
| Authors: | J Chen R Deaton M Garzon J -W Kim D H Wood H Bi D Carpenter Y -Z Wang |
| |
| Affiliation: | (1) Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA;(2) Computer Science and Engineering, University of Arkansas, Rm. 311 ENGR, Fayetteville, AR 72701, USA;(3) Computer Science, University of Memphis, Memphis, TN 38138, USA;(4) Biological Engineering, University of Arkansas, Fayetteville, AR 72701, USA;(5) Computer and Information Sciences, University of Delaware, Newark, DE 19716, USA |
| |
| Abstract: | Libraries of DNA oligonucleotides manufactured by an in vitro selection protocol were characterized for their non-crosshybridizing properties. Cloning and sequencing after several iterations
of the protocol showed that the sequences, in general, became more non-crosshybridizing. Gel electrophoresis of protocol product,
also, indicated non-crosshybridization, and showed evolution in the population of molecules under the non-crosshybridization
selection pressure. Melting curves of protocol product also indicated non-crosshybridization when compared to control samples.
Thus, it appears that the protocol does select populations of non-crosshybridizing sequences. |
| |
| Keywords: | DNA computing melting temperature nanotechnology word design |
| 本文献已被 SpringerLink 等数据库收录！ |
|
