Application of Mismatch Detection Methods in DNA Computing |
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| Authors: | Christiaan V Henkel Grzegorz Rozenberg Herman P Spaink |
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| Affiliation: | (1) Institute of Biology, Leiden University, Wassenaarseweg 64, 2333, AL, Leiden, The Netherlands;(2) Leiden Institute of Advanced Computer Science, Leiden University, Niels Bohrweg 1, 2333, CA, Leiden, The Netherlands |
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| Abstract: | In many implementations of DNA computing, reliable detection of hybridization is of prime importance. We have applied several
well-established DNA mutation scanning methods to this problem. Since they have been developed for speed and accuracy, these
technologies are very promising for DNA computing. We have benchmarked a heteroduplex migration assay and enzymatic detection
of mismatches on a 4 variable instance of 3SAT, using a previously described blocking algorithm. The first method is promising,
but yielded ambiguous results. On the other hand, we were able to distinguish all perfect from imperfect duplexes by means
of a CEL I mismatch endonuclease assay. |
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| Keywords: | DNA computing DNA hybridization mismatch detection mutation detection |
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