微氧条件下培养AOB-Anammox颗粒污泥

采用絮状硝化污泥和厌氧氨氧化颗粒污泥为接种污泥,利用膨胀颗粒污泥床反应器,在微氧曝气条件下,培养自养脱氮(氨氧化AOB-厌氧氨氧化Anammox)颗粒污泥.在无机高氨氮进水条件下,维持反应器运行58 d,成功培养出AOB-Anammox颗粒污泥.在模拟生活污水条件下,颗粒污泥脱氮效果稳定,氨氮和总氮去除率最高可达92.3%、71.2%,平均总氮去除负荷达1.237 kg·N/(m~3·d).SEM及FISH结果表明:AOB-Anammox颗粒污泥微生物组成以2种菌群为主,AOB细菌密集排布于颗粒污泥表面,Anammox细菌均匀分布在颗粒污泥内部.

Cultivation Process of AOB-Anammox Granular Sludge Under Micro-aerobic Condition

An expanded granular sludge bed ( EGSB ) reactor seeded with the mixture of flocculent nitrification sludge and anaerobic ammonium oxidation ( Anammox ) granular was started up to cultivate the ammonium oxidizing bacteria ( AOB)-Anammox granular sludge under micro-aerobic condition. The mature AOB-Anammox granular sludge was successfully formed after performing of the EGSB reactor for 58 days in terms of the high ammonia-nitrogen concentration and inorganic influent. Under the circumstance of synthetic domestic sewage, the performance of the sludge was testified and proven to be stable. The maximum removal rate of NH4+-N and total nitrogen ( TN ) reached 92. 3% and 71. 2%, respectively, along with the average nitrogen loading removal (NLR) reaching 1. 237 kg·N/(m3·d). Results of SEM and FISH analysis show that the AOB-Anammox granular sludge is mainly structured by two types of microflora. More specifically, AOB are configured on the surface of the sludge intensively while Anammox bacteria are homogeneously distributed inside the granular.