蜡状芽孢杆菌的筛选及其产氢性能

为了研究纯菌种对产氢的影响,从污水厂活性污泥中分离了纯的产氢菌种XN12,根据16S rDNA序列和DnaJ基因序列、细胞形态、生理生化数据等多项鉴定分析,该菌种被鉴定为蜡状芽孢杆菌(Bacillus cereus).为了研究该纯菌的产氢性能,实验以葡萄糖为底物,在不同pH值条件下观察其产气情况.实验结果表明:以每摩尔葡萄糖计,该菌在中性条件下获得最大产氢量为1.6 mol.对反应的液相产物的检验结果表明:该菌为丁酸发酵菌,通过丙酮酸代谢途径产出氢气.

Isolation of Bacillus cereus and Its Characterization on Hydrogen Production

To study the pure bacteria on producing hydrogen,one pure bacterium XN12 was isolated from active sludge of wastewater plant. Based on the sequencing of 16 S rDNA and DnaJ gene and cellular morphology as well as biochemical reactions,the isolates were identified as Bacillus cereus. In the condition of glucose as the substrate and with different operational pH values,the characterization on hydrogen yielding with the pure bacterium was studied. Results showed that for every mol glucose,optimum condition for maximizing H2 production was initial pH of 6. 9,in which the maximum H2 production was 1. 6 mol H2. According to the metabolic production of the reactions,the strain was butyric acid fermentation type and produced hydrogen through pyruvate metabolism pathway.