| 城市污水SBR法短程生物脱氮系统硝化菌群的定量分析 |
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| 引用本文： | 杨庆,彭永臻,曾薇,杨岸明,Hiroyasu Satoh,Takashi Mino.城市污水SBR法短程生物脱氮系统硝化菌群的定量分析[J].北京工业大学学报,2007,33(8):843-848. |
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| 作者姓名： | 杨庆 彭永臻 曾薇 杨岸明 Hiroyasu Satoh Takashi Mino |
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| 作者单位： | 北京工业大学,北京市水质科学与水环境恢复工程重点试验室,北京,100022;Institute of Environmental Studies, University of Tokyo, Tokyo 113-8656, Japan |
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| 基金项目： | 国家“八六三”重大科技专项资助项目(2004AA601020);;国家自然科学基金重大国际合作项目(50521140075);;“十一五”国家科技支撑计划(2006BAC19803);;国家自然科学基金(50608001)． |
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| 摘 要： | 为了研究实时控制对短程生物脱氮中试系统内硝化菌群结构组成的影响,在考察系统常温条件下处理城市污水时短程硝化效果的同时,采用FISH、PCR-DGGE和PCR-克隆序列(Cloning Sequencing)分子生物学方法对SBR中试系统(有效容积为54 m~3)硝化菌群中的氨氧化菌(AOB)和亚硝酸氧化菌(NOB)进行定性与定量化分析.FISH结果表明,在短程脱氮系统中,AOB相比于NOB已成为明显的优势菌群,占总菌群的3%～12%;且没有检测出NOB.PCR-DGGE结果表明,SBR短程脱氮中试系统中的AOB均以Nitrosomonas-like为主.污泥样品的PCR-Cloning-Sequencing结果表明,所有的克隆相似于Nitrosomonas,其中60%以上的克隆相似于Nitro- somonas europaea.
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| 关 键 词： | SBR 短程生物脱氮 Fish PCR-DGGE PCR-克隆序列 |
| 文章编号： | 0254-0037（2007）08-0843-06 |
| 修稿时间： | 2006-12-05 |
Quantitative Analysis of Nitrifying Communities in Short-cut Nitrogen Removal From Municipal Wastewater of SBR |
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| YANG Qing,PENG Yong-zhen,ZENG Wei,YANG An-ming,Hiroyasu Satoh,Takashi Mino.Quantitative Analysis of Nitrifying Communities in Short-cut Nitrogen Removal From Municipal Wastewater of SBR[J].Journal of Beijing Polytechnic University,2007,33(8):843-848. |
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| Authors: | YANG Qing PENG Yong-zhen ZENG Wei YANG An-ming Hiroyasu Satoh Takashi Mino |
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| Affiliation: | 1. Key Laboratory of Beijing Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100022, China; 2. Institute of Environmental Studies, University of Tokyo, Tokyo 113-8656, Japan |
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| Abstract: | To investigate the effects of real-time control on the structure of nitrifying communities of the short-cut nitrogen removal in SBR pilot-scale plant with a working volume of 54 m~3,molecular techniques of Fish,PCR-DGGE and PCR-Cloning-Sequencing were used to analyze ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) with the performance of short-cut nitrogen removal.Fish results show that in short-cut nitrogen removal process,AOB became dominant with compared to NOB and accounted for 3 %... |
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| Keywords: | SBR short-cut nitrification-denitrification Fish PCR-DGGE PCR-Cloning-Sequencing |
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