花斑无须鲶(Ageneiosus marmoratus)全基因组微卫星分布特征研究

根据NCBI已公布的花斑无须鲶全基因组序列,利用MISA软件对花斑无须鲶全基因组的6种完整型微卫星进行筛选并分析其分布特征. 结果如下:在花斑无须鲶全基因组(约1.03Gb)中符合条件的微卫星序列共336 037个,丰度为326个/Mb. 微卫星总长度为7 720 686 bp,占花斑无须鲶全基因组的0.75%. 其中二碱基类型的微卫星数目最多,为145 318个,占微卫星总数的43.24%,其次分别为单碱基(37.12%)、三碱基(11.00%)、四碱基(7.39%)、五碱基(1.04%)和六碱基(0.21%). 花斑无须鲶全基因组微卫星中的优势碱基类别按照数量从高到低排列依次为A、AC、AG、AT、AAT、AAAT、TATC、AAG、AAC和TGA,共有305 243个,占微卫星总数的 90.84%,A、T碱基在微卫星中占绝对优势.

Microsatellite Distribution in the Whole Genome of Ageneiosus marmoratus

According to the complete genome sequence of Ageneiosus marmoratus published on NCBI,the microsatellites of the whole genome of Ageneiosus marmoratus are screened and analyzed by using MISA software. The results are as follows:there are 336 037 microsatellite sequences in the whole genome of Ageneiosus marmoratus(about 1.03 Gb),and the abundance is 326/MB. The total length of microsatellites is 7 720 686 bp,accounting for 0.75% of the whole genome. Among them,the number of microsatellites with two bases is the most,which is 145 318,accounting for 43.24% of the total number of microsatellites,followed by single base(37.12%),triple base(11.00%),four base(7.39%),five base(1.04%)and six base(0.21%). The dominant base types of microsatellites in the whole genome of Ageneiosus marmoratus are A,AC,AG,AT,AAT,AAAT,TATC,AAG,AAC and TGA,with a total of 305 243,accounting for 90.84% of the total number of microsatellites.