| Comparative analysis of hydrogen-producing bacteria and its immobilized cells for characteristics of hydrogen production |
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| 作者姓名： | 王相晶 任南琪 向文胜 王爱杰 林明 郭婉茜 |
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| 作者单位： | School of Municipal and Environmental Engineering，Harbin Institute of Technology，School of Municipal and Environmental Engineering，Harbin Institute of Technology，Research Center of Life Science and Biotechnology，Northeast Agricultural University，School of Municipal and Environmental Engineering，Harbin Institute of Technology，School of Municipal and Environmental Engineering，Harbin Institute of Technology，School of Municipal and Environmental Engineering，Harbin Institute of Technology Harbin 150090，China，Harbin 150090，China，Harbin 150030，China，Harbin 150090，China，Harbin 150090，China，Harbin 150090，China |
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| 基金项目： | SponsoredbytheNational973ProjectofChina(GrantNo .G2 0 0 0 0 2 64 0 2 ) . |
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| 摘 要： | 0 INTRODUCTIONConcerningglobalenvironmentalimpacts ,suchasthegreenhouseeffectandresourcerecovery ,theprospectsofusinghydrogenasanalternativeandsustainableenergysourcehavecontributedtoanintensivestudyofbiohydro genproductionfromrenewablebiomass .ManystudiesonbiohydrogengenerationhavebeenreviewedbyNandietal1] andbyBenemann2 ] .Thefermentativehydrogenpro ductionhasalwaysbeenconfrontedwiththeproblemoflowrateofhydrogenproductioncomparedwithothertech niquesforproducinghydrogen .Thismaybesol…
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Comparative analysis of hydrogen-producing bacteria and its immobilized cells for characteristics of hydrogen production |
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| WANG Xiang-jing,REN Nan-qi,XIANG Wen-sheng,WANG Ai-jie,LIN Ming,GUO Wan-qian.Comparative analysis of hydrogen-producing bacteria and its immobilized cells for characteristics of hydrogen production[J].Journal of Harbin Institute of Technology,2003,10(4). |
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| Authors: | WANG Xiang-jing REN Nan-qi XIANG Wen-sheng WANG Ai-jie LIN Ming GUO Wan-qian |
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| Affiliation: | 1. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China
2. Research Center of Life Science and Biotechnology, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | A strain of hydrogen producing bacteria was immobilized by polyvinyl alcohol-boric acid method,with the addition of a small amount of calcium alginate. The immobilized cells were insensitive to the presence of traces of O2. Moreover, the immobilized cells increased both the evolution rate and the yield of hydrogen production. Batch experiments with a medium containing 10 g/L glucose demonstrated the yields of hydrogen production by the immobilized and free cells were 2.14 mol/mol glucose and 1.69 mol/mol glucose, respectively.In continuous cultures atmedium retention time of 2. 0 h, the yield and the evolution rate of hydrogen producmedium retention time of 6. 0 h, the yield and the evolution rate of hydrogen production by free cells were only 1.75 mol/mol glucose and 362.9ml/(L·h),respectively. |
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| Keywords: | hydrogen producing bacteria immobilized cells characteristics of hydrogen production |
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