| 牛奶中硫氰酸钠的检测方法研究 |
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| 引用本文： | 杜兴兰,徐向东,王景岩,仙保芹,刘向芳,李坤全.牛奶中硫氰酸钠的检测方法研究[J].山东轻工业学院学报,2012(2):27-29. |
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| 作者姓名： | 杜兴兰 徐向东 王景岩 仙保芹 刘向芳 李坤全 |
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| 作者单位： | 聊城市产品质量监督检验所 |
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| 基金项目： | 山东省质量技术监督局立项项目(2009KY10) |
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| 摘 要： | 牛奶中非法添加硫氰酸钠会对人体产生危害,但是目前的检测方法检出限偏高,探讨新的检测方法颇为重要。本文建立了牛奶中硫氰酸钠的高效液相色谱检测方法,先在样品中加入不同含量量的硫氰酸钠。样品经乙醇提取,然后过固相萃取柱,以十六烷基三甲基溴化铵(2 mmol/L)和柠檬酸(2 mmol/L)为流动相,用二极管阵列检测器检测,检测波长218 nm,该方法线性范围为0.2～100 ug/mL,所加硫氰酸钠的回收率为95%以上。所以这种方法准确可靠,精密度高。
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| 关 键 词： | 牛奶 硫氰酸钠 液相色谱 |
The Determination Of Sodium Thiocyanate In Milk By High Performance Liquid Chromatography Method |
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| DU Xing-lan,XU Xiang-dong,WANG Jing-yan,XIAN Bao-qin,LIU Xiang-fang,LI Kun-quan.The Determination Of Sodium Thiocyanate In Milk By High Performance Liquid Chromatography Method[J].Journal of Shandong Institute of Light Industry(Natural Science Edition),2012(2):27-29. |
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| Authors: | DU Xing-lan XU Xiang-dong WANG Jing-yan XIAN Bao-qin LIU Xiang-fang LI Kun-quan |
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| Affiliation: | (Liaocheng Institute Of Product Quality Supervision & Inspection Postalcode,Liaocheng 252000,China) |
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| Abstract: | The high performance liquid chromatography(HPLC) method was established for the detection of sodium thiocyanate(NaSCN) residues in milk samples.After the extraction by ethanol,NaSCN in the milk samples was determined by diode-array detector at a wavelength of 218 nm with the mobile phase containing 2 mmol/L of cetyl trimethyl ammonium bromide(CTAB) and 2 mmol/L of citric acid.The linear range of this detection is 0.4~100 ug/mL.The addition standard recovery is more than 95%.The method is accurate,reproducible,and suitable for quality control. |
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| Keywords: | milk NaSCN high performance liquid chromatography |
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